a. To determine: The technique which can be used to identify specific miRNA that might be associated with the discovery of the protein. Introduction: miRNA refers to non-coding RNA that play a major role in the regulation of gene expression. Different techniques can be used to detect specific miRNA that is associated with a particular protein molecule.

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Answer 1

Question

Chapter 16, Problem 27P

Summary Introduction

a.

To determine:

The technique which can be used to identify specific miRNA that might be associated with the discovery of the protein.

Introduction:

miRNA refers to non-coding RNA that play a major role in the regulation of gene expression. Different techniques can be used to detect specific miRNA that is associated with a particular protein molecule.

Summary Introduction

b.

To determine:

The ways by which mutant mouse can be used to identify the mRNA that might be targeted by the miRNA- RISC complexes containing Argonaute like protein.

Introduction:

The human genome contains 1000 genes encoding the miRNAs. The miRNAs become incorporated into ribonucleoprotein complexes called miRNA induced silencing complexes.

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Consider the now dominant variant of the SARS-CoV-2 called the D614G mutation: a) The mutation changes an Aspartate (D, Asp) to a Glycine (G, Gly) at nucleotide position 614 (that’s why it’s called the D614G mutant) in the S1 subunit of the Spike protein. Using only the information above and a codon table, what are the mRNA codon sequences of the 2019-dominant and 2020-dominant Spike proteins? Note the figure above is not needed toanswer the question. Report in 5’ to 3' orientation __________________________________________ b) What type of substitution is this? In your answer, address the following: • The expected substitution in base sequence (e.g., A à C)• If the mutation is synonymous, nonsynonymous, or a frameshift• If the mutation is a transition or a transversion c) The mutation increases infectivity by reducing the stability of the Spike protein such that it can remain in the open conformation more often. The open conformation increases the chances of binding to the host ACE2…

(b) Hemoglobin is made of B-globin subunits. The first few mRNA nucleotides for B- globin are given by: (1) (iii) (iv) Write down the DNA sequence that has led to this mRNA and indicate the sense and non-sense strands and the polarity. CE Derive the polypeptide for the sequence using the table of the genetic code (Table Q1 below) and indicate the polarity of the polypeptide chain. First Position (5' end) U A single point mutation in mRNA sequence can cause sickle cell anemia by changing the amino acid Glu to Val. For the given mRNA, indicate the point mutations for the first Glu in the polypeptide sequence that can cause this disease. 5'-AUGGUCCACCUGACUCCUGAGGAGAAG...UGA-3' C The polypeptide of B-globin contains the amino acid Leu. Write down all the anticodons of the tRNA molecules that can potentially code for Val. Indicate the polarity of the anti-codon. A G Table 1. The Codons of the Genetic Code Second Position U Phe Phe Leu Leu Leu Leu Leu Leu Ile Ile Ile Met-Start Val Val Val…

Consider the following mRNA base sequence 5' CUG-CAC 3' (a) What dipeptide is coded for by this mRNA? (b) What dipeptide is formed if a mutation converts CUG to CUU? (c) What dipeptide is formed if a mutation converts CAC to CGC? (d) What dipeptide is formed if a mutation converts CUG to CUU and CAC to CGC?

Answer 2

Question

Chapter 16, Problem 27P

Summary Introduction

a.

To determine:

The technique which can be used to identify specific miRNA that might be associated with the discovery of the protein.

Introduction:

miRNA refers to non-coding RNA that play a major role in the regulation of gene expression. Different techniques can be used to detect specific miRNA that is associated with a particular protein molecule.

Summary Introduction

b.

To determine:

The ways by which mutant mouse can be used to identify the mRNA that might be targeted by the miRNA- RISC complexes containing Argonaute like protein.

Introduction:

The human genome contains 1000 genes encoding the miRNAs. The miRNAs become incorporated into ribonucleoprotein complexes called miRNA induced silencing complexes.

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Answer 3

Question

Chapter 16, Problem 27P

Summary Introduction

a.

To determine:

The technique which can be used to identify specific miRNA that might be associated with the discovery of the protein.

Introduction:

miRNA refers to non-coding RNA that play a major role in the regulation of gene expression. Different techniques can be used to detect specific miRNA that is associated with a particular protein molecule.

Summary Introduction

b.

To determine:

The ways by which mutant mouse can be used to identify the mRNA that might be targeted by the miRNA- RISC complexes containing Argonaute like protein.

Introduction:

The human genome contains 1000 genes encoding the miRNAs. The miRNAs become incorporated into ribonucleoprotein complexes called miRNA induced silencing complexes.

Expert Solution & Answer

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Answer 4

Question

Chapter 16, Problem 27P

Summary Introduction

a.

To determine:

The technique which can be used to identify specific miRNA that might be associated with the discovery of the protein.

Introduction:

miRNA refers to non-coding RNA that play a major role in the regulation of gene expression. Different techniques can be used to detect specific miRNA that is associated with a particular protein molecule.

Summary Introduction

b.

To determine:

The ways by which mutant mouse can be used to identify the mRNA that might be targeted by the miRNA- RISC complexes containing Argonaute like protein.

Introduction:

The human genome contains 1000 genes encoding the miRNAs. The miRNAs become incorporated into ribonucleoprotein complexes called miRNA induced silencing complexes.

Expert Solution & Answer

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Answer 5

Chapter 16, Problem 27P

Summary Introduction

a.

To determine:

The technique which can be used to identify specific miRNA that might be associated with the discovery of the protein.

Introduction:

miRNA refers to non-coding RNA that play a major role in the regulation of gene expression. Different techniques can be used to detect specific miRNA that is associated with a particular protein molecule.

Summary Introduction

b.

To determine:

The ways by which mutant mouse can be used to identify the mRNA that might be targeted by the miRNA- RISC complexes containing Argonaute like protein.

Introduction:

The human genome contains 1000 genes encoding the miRNAs. The miRNAs become incorporated into ribonucleoprotein complexes called miRNA induced silencing complexes.

Answer 6

Chapter 16, Problem 27P

Summary Introduction

a.

To determine:

The technique which can be used to identify specific miRNA that might be associated with the discovery of the protein.

Introduction:

miRNA refers to non-coding RNA that play a major role in the regulation of gene expression. Different techniques can be used to detect specific miRNA that is associated with a particular protein molecule.

Answer 7

Chapter 16, Problem 27P

Summary Introduction

a.

To determine:

The technique which can be used to identify specific miRNA that might be associated with the discovery of the protein.

Introduction:

miRNA refers to non-coding RNA that play a major role in the regulation of gene expression. Different techniques can be used to detect specific miRNA that is associated with a particular protein molecule.

Answer 8

Summary Introduction

b.

To determine:

The ways by which mutant mouse can be used to identify the mRNA that might be targeted by the miRNA- RISC complexes containing Argonaute like protein.

Introduction:

The human genome contains 1000 genes encoding the miRNAs. The miRNAs become incorporated into ribonucleoprotein complexes called miRNA induced silencing complexes.

Answer 9

Summary Introduction

b.

To determine:

The ways by which mutant mouse can be used to identify the mRNA that might be targeted by the miRNA- RISC complexes containing Argonaute like protein.

Introduction:

The human genome contains 1000 genes encoding the miRNAs. The miRNAs become incorporated into ribonucleoprotein complexes called miRNA induced silencing complexes.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 16 - For each of the terms in the left column, choose...Ch. 16 - Does each of the following types of gene...Ch. 16 - List five events other than transcription...Ch. 16 - Which eukaryotic RNA polymerase RNA pol I, pol II,...Ch. 16 - You have synthesized an enhancerless GFP reporter...Ch. 16 - Prob. 6P Ch. 16 - Yeast genes have cis-acting elements upstream of...Ch. 16 - A single UASG regulates the expression of three...Ch. 16 - Prob. 9P Ch. 16 - a. Assume that two transcription factors are...

Solution Summary: The author explains how different techniques can be used to identify specific miRNA that is associated with a particular protein molecule.

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