Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Chapter 16, Problem 11P
| a. | You want to create a genetic construct that will express GFP in Drosophila. In addition to the GFP coding sequence, what DNA element(s) must you include in order to express this protein in flies if the construct were integrated into the Drosophila genome? Where should such DNA element(s) be located? How would you ensure that GFP is expressed only in certain tissues of the fly, such as the wing? |
| b. | Suppose you insert the GFP coding region plus all of the DNA elements required by the answer to part (a) excepting the enhancer in between inverted repeats found at the end of a particular transposable element. Because all of the DNA sequences located between these inverted repeats can move from place to place in the Drosophila genome, you can generate many different fly strains, each with the construct integrated at a different genomic location. You now examine animals from each strain for GFP fluorescence. Animals from different strains show different patterns: some glow green in the eyes, others in legs, some show no green fluorescence, etc. Explain these results and describe a potential use for this experiment. |
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12. a. You want to create a genetic construct that will express GFP in Drosophila. In addition to the GFPcoding sequence, what DNA element(s) must youinclude in order to express this protein in flies if theconstruct were integrated into the Drosophila genome? Where should such DNA element(s) be located? How would you ensure that GFP is expressedonly in certain tissues of the fly, such as the wing?b. Suppose you insert the GFP coding region plus allof the DNA elements required by the answer to part(a)—except the enhancer—between inverted repeatsfound at the ends of a particular transposable element.Because all of the DNA sequences located betweenthese inverted repeats can move from place to placein the Drosophila genome, you can generate manydifferent fly strains, each with the construct integrated at a different genomic location. You now examine animals from each strain for GFPfluorescence. Animals from different strains showdifferent patterns: some glow green in the eyes,others in…
Protein levels and mRNA levels for a particualr gene don’t always match. For example, the GCN4 gene in yeast is always producing mRNA, but the Gcn4 protein is only made when the cells are starved.
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2. a. You want to create a genetic construct that will express GFP in Drosophila. In addition to the GFPcoding sequence, what DNA element(s) must youinclude in order to express this protein in flies if theconstruct were integrated into the Drosophila genome? Where should such DNA element(s) be located? How would you ensure that GFP is expressedonly in certain tissues of the fly, such as the wing?b. Suppose you insert the GFP coding region plus allof the DNA elements required by the answer to part(a)—except the enhancer—between inverted repeatsfound at the ends of a particular transposable element.Because all of the DNA sequences located betweenthese inverted repeats can move from place to placein the Drosophila genome, you can generate manydifferent fly strains, each with the construct integrated at a different genomic location. You now examine animals from each strain for GFPfluorescence. Animals from different strains showdifferent patterns: some glow green in the eyes,others in…
Chapter 16 Solutions
Genetics: From Genes to Genomes, 5th edition
Ch. 16 - For each of the terms in the left column, choose...Ch. 16 - Does each of the following types of gene...Ch. 16 - List five events other than transcription...Ch. 16 - Which eukaryotic RNA polymerase RNA pol I, pol II,...Ch. 16 - You have synthesized an enhancerless GFP reporter...Ch. 16 - Prob. 6PCh. 16 - Yeast genes have cis-acting elements upstream of...Ch. 16 - A single UASG regulates the expression of three...Ch. 16 - Prob. 9PCh. 16 - a. Assume that two transcription factors are...
Ch. 16 - a. You want to create a genetic construct that...Ch. 16 - In Problem 12, you identified a genomic region...Ch. 16 - Prob. 13PCh. 16 - Prob. 14PCh. 16 - Genes in both prokaryotes and eukaryotes are...Ch. 16 - Prob. 16PCh. 16 - Prader-Willi syndrome is caused by a mutation in...Ch. 16 - The human IGF-2R gene is autosomal and maternally...Ch. 16 - Follow the expression of a paternally imprinted...Ch. 16 - Reciprocal crosses were performed using two inbred...Ch. 16 - Interestingly, imprinting can be tissue-specific....Ch. 16 - Prob. 22PCh. 16 - a. How can a single eukaryotic gene give rise to...Ch. 16 - A hunchback gene, a gene necessary for proper...Ch. 16 - You know that the mRNA and protein produced by a...Ch. 16 - You are studying a transgenic mouse strain that...Ch. 16 - Prob. 27PCh. 16 - Scientists have exploited the siRNA pathway to...Ch. 16 - Researchers know that Fru-M controls male sexual...Ch. 16 - The Drosophila gene Sex lethal Sxl is deserving of...Ch. 16 - Prob. 31P
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