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Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Question
Chapter 16, Problem 22P
Summary Introduction
To determine:
The way by which the antibodies (that bind specifically to DNA fragments containing 5-methylcytosine, but not to DNA lacking modified
Introduction:
The antibody or immunoglobin is a protein which is used by the immune system to neutralize or destroy the pathogen. It is produced by plasma cells of the body. A ChIp seq (ChIP-sequencing) is a technique used to analyze the interaction of proteins.
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A research group collaborating with the hospital extracted DNA from the peripheral blood leucocytes of the patient V.1, her sister V.2, her mother IV.1 and her
father IV.2 with consent and ethical approval for experimental work involving human tissues. These specimens were used for sequencing studies to screen for
causative variants in amyloid precursor protein (APP), presenilin-1 (PSEN1) and presenlin-2 (PSEN-2) genes. The outcome is shown in Fig. 2 below.
APP
IV.1 961 TACGGCGGATGTGGCGGCAACCGGAACAACTTTGACACAGAAGAGTACTGCATGGCCGTG
V.2 961 TACGGCGGATGTGGCGGCAACCGGAACAACTTTGACACAGAAGAGTACTGCATGGCCGTG
-Y--G--G--C--G--G--N--R--N--N--F--D--T--E--E--Y--C--M--A--V-
Amino
Acid
IV.2 961 TACGGCGGATGTGGCGGCAACCGGAATAACTTTGACACAGAAGAGTACTGCATGGCCGTG 1020
TACGGCGGATGTGGCGGCAACCGGAATAACTTTGACACAGAAGAGTACTGCATGGCCGTG 1020
Amino Acid -YGGCGGNRN NFD TEEY C-M-A-V-340
V.1
961
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IV.1 361
V.2 361
Amino Acid
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1020
1020
340
CATACCGACACTCTCCCCCACACACCCCTGCACTCAATTCTCAATCCTCCCATCATCATC 420…
Bacteriophage P22 was used in generalised transduction experiments to infect the Salmonella
typhimurium donor strains described in the table below.
The resulting phage lysates were then used to infect the recipient strains of S. typhimurium
recipient strains listed in the table.
In each cross, a phenotype was selected for one of the selected for one of the three genetic
markers studied (str, aceA, thrA), and were made to select the recombinants corresponding to the
other two markers. markers.
The results are given in the following table:
Strain
I donor
str thrA
aceA thrA
str aceA+
Strain
recipient
strs thrA+
aceA thrA
str aceA
Phenotype
selected
Str
Ace+
Str
recombinants
selected
ThrA
ThrA
ThrA
ThrA
Ace
Ace
Number
60
40
95
5
10
90
str: gene involved in streptomycin resistance,
aceA: gene involved in the use of acetate as a carbon source,
thrA: gene involved in threonine biosynthesis.
1) What are the selective media used in these three transduction experiments? to obtain the
selected…
A research group is studying a bacterium X that binds to mucosal cells in the lung and invades. Wildtype X has an LD50 value of 10 bacteria when administered to mice by inhalation. Using transposon mutagenesis, the researchers have isolated two mutants of X that they call Xmut1 and Xmut2, both of which have LD50 values of 105 when inhaled by mice. However, in tissue culture cells, Xmut1 can invade the cells just as well as wild-type X, while Xmut2 cannot. Provide a possible explanation for these results.
Chapter 16 Solutions
Genetics: From Genes to Genomes, 5th edition
Ch. 16 - For each of the terms in the left column, choose...Ch. 16 - Does each of the following types of gene...Ch. 16 - List five events other than transcription...Ch. 16 - Which eukaryotic RNA polymerase RNA pol I, pol II,...Ch. 16 - You have synthesized an enhancerless GFP reporter...Ch. 16 - Prob. 6PCh. 16 - Yeast genes have cis-acting elements upstream of...Ch. 16 - A single UASG regulates the expression of three...Ch. 16 - Prob. 9PCh. 16 - a. Assume that two transcription factors are...
Ch. 16 - a. You want to create a genetic construct that...Ch. 16 - In Problem 12, you identified a genomic region...Ch. 16 - Prob. 13PCh. 16 - Prob. 14PCh. 16 - Genes in both prokaryotes and eukaryotes are...Ch. 16 - Prob. 16PCh. 16 - Prader-Willi syndrome is caused by a mutation in...Ch. 16 - The human IGF-2R gene is autosomal and maternally...Ch. 16 - Follow the expression of a paternally imprinted...Ch. 16 - Reciprocal crosses were performed using two inbred...Ch. 16 - Interestingly, imprinting can be tissue-specific....Ch. 16 - Prob. 22PCh. 16 - a. How can a single eukaryotic gene give rise to...Ch. 16 - A hunchback gene, a gene necessary for proper...Ch. 16 - You know that the mRNA and protein produced by a...Ch. 16 - You are studying a transgenic mouse strain that...Ch. 16 - Prob. 27PCh. 16 - Scientists have exploited the siRNA pathway to...Ch. 16 - Researchers know that Fru-M controls male sexual...Ch. 16 - The Drosophila gene Sex lethal Sxl is deserving of...Ch. 16 - Prob. 31P
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- Bacteriophage P22 was used in generalized transduction experiments to infect the Salmonella typhimurium donor strains described in the table below. The resulting phage lysates were then used to infect the S. typhimurium recipient strains listed in the table. In each cross, a phenotype was selected for one of the three genetic markers studied (str, aceA, thrA), and then replicates were performed to select the corresponding recombinants for the other two markers. The results are given in the following table: Recipient strain Selected phenotype Selected recombinants Donor strain str thrA aceA+ thrA str aceA+ strs thrA+ aceA thrA+ str aceA Str Ace+ Str ThrA ThrA+ ThrA ThrA+ Ace Ace str: gene involved in streptomycin resistance, aceA gene involved in the use of acetate as a carbon source, thrA: gene involved in the biosynthesis of threonine. Number 60 40 95 5 10 90 Determine the order of the genes and draw a genetic map showing this orderarrow_forwardWith respect to recombinant vaccines, it is CORRECT to state that: * (Only one statement is correct) (a) Protein subunit vaccines are formulated with the expression vector encoding the immunogenic protein or protein fragment (b) Recombinant carrier organisms are genetically modified to contain the pathogenic protein of the organism that will be the target of the vaccine (c) Nucleic acid vaccines are formulated with DNA or RNA molecules that, once internalized by cells, will generate recombinant proteins that will trigger the immune response against the desired pathogen (d) Unlike DNA vaccines, mRNA vaccines do not need to be internalized by cells, being recognized as foreign molecules to the organism that received them. (e) Laboratory-produced phages can be used as delivery tools in DNA vaccines, which will allow the expression of immunogenic proteins to the organism that received them (f) Pseudoviral particles (VLPs) are produced in cells that receive expression vectors…arrow_forwardDraw a hairpin structure like that shown in Figure 18.5 for the repeated sequence found in fragile-X syndrome (see Table 18.1).arrow_forward
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