Essentials Of Genetics (9th Global Edition)
9th Edition
ISBN: 9780134143637
Author: William S. Klug, Michael R. Cummings
Publisher: Pearson Global Edition
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Chapter 17, Problem 11PDQ
Summary Introduction
To review:
Use of probe in recombinant DNA technology.
Introduction:
Recombinant DNA technology involves cutting and joining of DNA segments from different sources by restriction enzymes and DNA ligase. It involves digesting a vector DNA with the help of restriction enzyme that cuts on specific DNA sites. The probe is a fragment of a short stretch of DNA, which is radioactively labeled. It follows hybridization method for the identification of DNA and RNA
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Chapter 17 Solutions
Essentials Of Genetics (9th Global Edition)
Ch. 17 -
CASE STUDY |Should we worry about recombinant DNA...Ch. 17 - Prob. 2CSCh. 17 - Prob. 3CSCh. 17 -
HOW DO WE KNOW?
1. In this chapter we focused on...Ch. 17 - Prob. 2PDQCh. 17 - What roles do restriction enzymes, vectors, and...Ch. 17 - Prob. 4PDQCh. 17 - Prob. 5PDQCh. 17 - Prob. 6PDQCh. 17 - Prob. 7PDQ
Ch. 17 - List the advantages and disadvantages of using...Ch. 17 - What are the advantages of using a restriction...Ch. 17 - The introduction of genes into plants is a common...Ch. 17 - Prob. 11PDQCh. 17 - Prob. 12PDQCh. 17 - Prob. 13PDQCh. 17 - What advantages do cDNA libraries provide over...Ch. 17 - Prob. 15PDQCh. 17 -
16. List the steps involved in screening a...Ch. 17 -
17. In a typical PCR reaction, describe what is...Ch. 17 -
18. We usually think of enzymes as being most...Ch. 17 - How are dideoxynucleotides (ddNTPs) structurally...Ch. 17 - Prob. 20PDQCh. 17 - One complication of making a transgenic animal is...Ch. 17 -
22. When disrupting a mouse gene by knockout, why...Ch. 17 - Prob. 23PDQ
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- What are some general “requirements” for recombinant DNA technology?arrow_forwardWhat are the advantages and disadvantages of microarraytechnology compared to sequencing PCR products?arrow_forwardWould it be appropriate to use DNA probes such as VNTR in DNA fingerprinting of an bacteriophage?arrow_forward
- explain the uses of the polymerase chain reaction, gel electrophoresis, and DNA probes, and how they work?arrow_forwardBriefly Explain the role of restriction enzymes in recombinant DNA technology. Please explain at your own words.arrow_forwardWhat are the differences between preparing an RNA sample for sequencing and a genomic DNA sample?arrow_forward
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