Essentials Of Genetics (9th Global Edition)
9th Edition
ISBN: 9780134143637
Author: William S. Klug, Michael R. Cummings
Publisher: Pearson Global Edition
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Chapter 17, Problem 4PDQ
Summary Introduction
To review:
The mechanism by which a gene that is modified by mRNA processing, can be cloned to produce insulin.
Introduction:
Recombinant protein produced by transferring the gene into the selected host cell with the help of cloning experiments and genetic engineering, thus leads to the generation of recombinant protein. Recombinant protein synthesis in a host cell is based on recombinant DNA technology (RDT).
Moreover, a recombinant protein is an important component of pharmaceuticals and thus leads to the production of recombinant protein at a large scale in the host cell (bacterial cell system, mammalian cell culture etc.) and a number of useful products can synthesize by this technique like human growth hormone, erythropoietin, and blood clotting factors, tissue plasminogen activator and insulin, which show therapeutic use for human welfare.
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Chapter 17 Solutions
Essentials Of Genetics (9th Global Edition)
Ch. 17 -
CASE STUDY |Should we worry about recombinant DNA...Ch. 17 - Prob. 2CSCh. 17 - Prob. 3CSCh. 17 -
HOW DO WE KNOW?
1. In this chapter we focused on...Ch. 17 - Prob. 2PDQCh. 17 - What roles do restriction enzymes, vectors, and...Ch. 17 - Prob. 4PDQCh. 17 - Prob. 5PDQCh. 17 - Prob. 6PDQCh. 17 - Prob. 7PDQ
Ch. 17 - List the advantages and disadvantages of using...Ch. 17 - What are the advantages of using a restriction...Ch. 17 - The introduction of genes into plants is a common...Ch. 17 - Prob. 11PDQCh. 17 - Prob. 12PDQCh. 17 - Prob. 13PDQCh. 17 - What advantages do cDNA libraries provide over...Ch. 17 - Prob. 15PDQCh. 17 -
16. List the steps involved in screening a...Ch. 17 -
17. In a typical PCR reaction, describe what is...Ch. 17 -
18. We usually think of enzymes as being most...Ch. 17 - How are dideoxynucleotides (ddNTPs) structurally...Ch. 17 - Prob. 20PDQCh. 17 - One complication of making a transgenic animal is...Ch. 17 -
22. When disrupting a mouse gene by knockout, why...Ch. 17 - Prob. 23PDQ
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- In order to manufacture insulin for patients with diabetes, scientists create recombinant DNA by inserting a human insulin gene into bacterial DNA and have the bacteria produce human insulin. Based on what you have learned in this unit about gene expression, what must the scientists do to the DNA sequence of the human insulin gene BEFORE inserting it into the bacteria, in order to have them produce the same insulin sequence that humans would produce? Explain.arrow_forwardThe human insulin gene contains a number of sequences thatare removed in the processing of the mRNA transcript. Bacterialcells cannot excise these sequences from mRNA transcripts, yetthis gene can be cloned into a bacterial cell and produce insulin.Explain how this is possible.arrow_forwardThe following is the only intron sequence of a gene that will be excised during the maturation of the mRNA. But it is not spliced in some tissues, where alternative splicing pattern is seen. Will the amino acid of its protein product following this sequence change? Explain with an example. ATAAGCCAGACTCAGCAarrow_forward
- The gene encoding the E. coli enzyme enolase begins with the sequence ATGTCCAAAATCGTA. What is the sequence of the RNA transcript specified by this part of the gene?arrow_forwardIn eukaryotes there is not a consistent relationship between the length of the coding sequence of a gene and the length of the mature mRNA it encodes, even though one nucleotide in DNA = one nucleotide in pre-mRNA or primary transcript. Explain why this is so.arrow_forwardMessenger RNA molecules are very difficult to isolate in bacteria because they are rather quickly degraded in the cell. Can you suggest a reason why this occurs? Eukaryotic mRNAs are more stable and exist longer in the cell than do bacterial mRNAs. Is this an advantage or a disadvantage for a pancreatic cell making large quantities of insulin?arrow_forward
- Protein levels and mRNA levels for a particualr gene don’t always match. For example, the GCN4 gene in yeast is always producing mRNA, but the Gcn4 protein is only made when the cells are starved. A. what is one mechanism by which changes in mRNA levels are not always matched by changes in the protein product of that mRNA?arrow_forwardGiven the DNA sequence TATAGCACTGAT, which of the following is the correct complementary mRNA sequence that would be produced? TUTUCGUGUCTU AUAUCGUGACUA ATATCGTGACTA CGCATGTCAGC None of the abovearrow_forwardA strain of bacteria possesses a temperature-sensitive mutation in the gene that encodes the rho subunit. At high temperatures, rho is not functional. When these bacteria are raised at elevated temperatures, which of the following effects would you expect to see? Explain your reasoning for accepting or rejecting each of these five options. a. Transcription does not take place. b. All RNA molecules are shorter than normal. c. All RNA molecules are longer than normal. d. Some RNA molecules are longer than normal. e. RNA is copied from both DNA strands.arrow_forward
- Human histone H1 protein has the protein sequence as shown as “KKASKPKKAAS- KAPTKKPKATPVKKAKKKLAATPKKAKKPKTVKAPVKASKPKKAKPVK” Please propose pathway the degradation of this protein.arrow_forwardMutation of codon 34 (CAC to GAC) and 85 (CGT to CAT) of the insulin gene causes hyperinsulinemia which may contribute to diabetes. Identify the amino acid replacement from these mutations. mia which may contribute to diabetes. Identify the amino acid replacement from these mutations.arrow_forwardThe length of a particular gene in human DNA, measured from the start site for transcription to the end of the protein-coding region, is 10,000 nucleotides, whereas the length of the mRNA produced from this gene is 4000 nucleotides. What is the most likely reason for this difference?arrow_forward
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