Chapter 17, Problem 7PDQ

Summary Introduction

To review:

The advantages of palindromic restriction sites in the DNA (deoxyribonucleic acid) strand.

Introduction:

Recombinant DNA technology (RDT) creates a combination of DNA segments by cutting and joining of DNA molecule from different sources. RDT is used to clone a small segment of DNA called DNA of interest. These small DNA segments are inserted into the vector for amplification of DNA molecule. DNA insert, vectors, DNA ligase, restriction enzymes, phosphatases or kinases, and host cell are the tools, which are involved in RDT. DNA strand is cleaved at the sugar-phosphate back bone by restriction enzymes.