Essentials Of Genetics (9th Global Edition)
9th Edition
ISBN: 9780134143637
Author: William S. Klug, Michael R. Cummings
Publisher: Pearson Global Edition
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Chapter 17, Problem 3CS
Summary Introduction
To review:
The risk associated with doing gene splicing experiment by an amateur in middle and high school biology courses.
Introduction:
RDT (Recombinant DNA technology) is the manipulation of DNA by cutting, joining, and ligation process. It follows to create a combination of DNA sequences from different sources. By analyzing, sequencing, and manipulation of the gene at the genetic level, promotes the success of creating recombinant clones. Moreover, the use of RDT proves as an effective measure to treat different genetic disorders, identification of novel genes, vaccine formation, and crop improvement by genetically modified organisms (GMOs).
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1a-Consider a situation where the primer sequences used in a PCR reaction are synthesized to be complementary to a mutation in the human genome that leads to a genetic disorder. Which of the following statements is true?
a- The PCR reaction will work if you use template DNA from someone who has the disorder.
b- The PCR reaction will work if you use template DNA from someone who does not have the disorder.
1b- In the diagram attached, can the jellyfish DNA be directly treated with DNA ligase so that its genome is reassembled and lacks the GFP gene?
a- No
b- Yes
Today, it is easy to make transgenic plants and animals. What are some important safety and ethical issues raised by this use of recombinant DNA technology? Explain your answer.
1A)
Which of the following best explains Griffith’s transformation experiments?
Two strains of S. pneumoniae were used for the experiment. Griffith injected a mouse with heat-inactivated S strain (pathogenic) and living R strain (non-pathogenic). The mouse died and living S strain was recovered from the dead mouse. From this, it was later concluded that that mutation occurred in the DNA of the R-strain cells thus transforming them into a pathogenic S strain.
Two strains of S. pneumoniae were used for the experiment. Griffith injected a mouse with heat-inactivated S strain (pathogenic) and living R strain (non-pathogenic). The mouse died and living S strain was recovered from the dead mouse. From this observation, it was later concluded that external DNA from the inactivated S strain was taken up by the R-strain cells thus transforming them into a pathogenic S strain.
Two strains of S. pneumoniae were used for the experiment. Griffith injected a mouse…
Chapter 17 Solutions
Essentials Of Genetics (9th Global Edition)
Ch. 17 -
CASE STUDY |Should we worry about recombinant DNA...Ch. 17 - Prob. 2CSCh. 17 - Prob. 3CSCh. 17 -
HOW DO WE KNOW?
1. In this chapter we focused on...Ch. 17 - Prob. 2PDQCh. 17 - What roles do restriction enzymes, vectors, and...Ch. 17 - Prob. 4PDQCh. 17 - Prob. 5PDQCh. 17 - Prob. 6PDQCh. 17 - Prob. 7PDQ
Ch. 17 - List the advantages and disadvantages of using...Ch. 17 - What are the advantages of using a restriction...Ch. 17 - The introduction of genes into plants is a common...Ch. 17 - Prob. 11PDQCh. 17 - Prob. 12PDQCh. 17 - Prob. 13PDQCh. 17 - What advantages do cDNA libraries provide over...Ch. 17 - Prob. 15PDQCh. 17 -
16. List the steps involved in screening a...Ch. 17 -
17. In a typical PCR reaction, describe what is...Ch. 17 -
18. We usually think of enzymes as being most...Ch. 17 - How are dideoxynucleotides (ddNTPs) structurally...Ch. 17 - Prob. 20PDQCh. 17 - One complication of making a transgenic animal is...Ch. 17 -
22. When disrupting a mouse gene by knockout, why...Ch. 17 - Prob. 23PDQ
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- LO 64- Explain how Next gen sequencing is applied in different technologies In which of the following scenario would it be best to use Next-gen sequencing? (select all that apply) a-To separate fragments of DNA based on their size- b-To insert a mutation at random in a gene c-To idently a novel pathogen d-To determine the nucleotide sequence of a microbe e- To insert methyl groups to cytosines in a DNA sequence asap pleasearrow_forwarda) What are vectors? Describe extensively the roles vectors play in genetic engineering? Write short notees on the following: Recombinant DNA, Cloning b) What are restriction enzymes? Describe extensively the roles restriction enzymes play in genetic engineering? Write short notees on the following: Selectable markers, Cloningarrow_forwardGenome comparisons have suggested that mouse DNA has mutated about twice as fast as human DNA. What is a possible explanation for this discrepancy? a. Mice are much smaller than humans. b. Mice live in much less sanitary conditions than humans and are therefore exposed to a wider range of mutation-causing substances. c. Mice have a smaller genome size. d. Mice have a much shorter generation time.arrow_forward
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