Essentials Of Genetics (9th Global Edition)
9th Edition
ISBN: 9780134143637
Author: William S. Klug, Michael R. Cummings
Publisher: Pearson Global Edition
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Chapter 17, Problem 17PDQ
In a typical PCR reaction, describe what is happening in stages occurring at temperature ranges (a) 90–95°C, (b) 50–70°C, and (c) 70–75°C.
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In a typical PCR reaction, describe what is happening in stages occurring at temperature ranges (a) 92–95°C, (b) 45–65°C, and (c) 65–75°C.
In a typical PCR reaction what is happening in stages occurring at temperature ranges (a) 92–95°C, (b) 45–65°C, and (c) 65–75°C.
You have a 100 micromolar stock solution of dNTPs in the freezer and are setting up a PCR reaction in which you need to have a final concentration of 20 micromolar dNTPs. In a reaction volume of 25 microliters, how much of your stock dNTPs do you add to each PCR reaction?
Chapter 17 Solutions
Essentials Of Genetics (9th Global Edition)
Ch. 17 -
CASE STUDY |Should we worry about recombinant DNA...Ch. 17 - Prob. 2CSCh. 17 - Prob. 3CSCh. 17 -
HOW DO WE KNOW?
1. In this chapter we focused on...Ch. 17 - Prob. 2PDQCh. 17 - What roles do restriction enzymes, vectors, and...Ch. 17 - Prob. 4PDQCh. 17 - Prob. 5PDQCh. 17 - Prob. 6PDQCh. 17 - Prob. 7PDQ
Ch. 17 - List the advantages and disadvantages of using...Ch. 17 - What are the advantages of using a restriction...Ch. 17 - The introduction of genes into plants is a common...Ch. 17 - Prob. 11PDQCh. 17 - Prob. 12PDQCh. 17 - Prob. 13PDQCh. 17 - What advantages do cDNA libraries provide over...Ch. 17 - Prob. 15PDQCh. 17 -
16. List the steps involved in screening a...Ch. 17 -
17. In a typical PCR reaction, describe what is...Ch. 17 -
18. We usually think of enzymes as being most...Ch. 17 - How are dideoxynucleotides (ddNTPs) structurally...Ch. 17 - Prob. 20PDQCh. 17 - One complication of making a transgenic animal is...Ch. 17 -
22. When disrupting a mouse gene by knockout, why...Ch. 17 - Prob. 23PDQ
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- . In the PCR process, if we assume that each cycle takes5 minutes, how manyfold amplification would be accomplished in 1 hour?arrow_forwardIn PCR amplification Why is it important to know the length of the sequence you amplify?arrow_forwardIf you wanted to prepare only one PCR reaction, how much of each reagent would you add to the PCR tube?] What is the final volume of the individual PCR reactions we are making?arrow_forward
- If you are trying to set up a PCR with a total volume of 40uL and you have access to a stock solution of 5x buffer, how much 5x buffer will you be adding to the PCR? ___uL?arrow_forwardThe best combination of regeants for a typical PCR reacion would include the following concentration of reagents: a. 1x buffer, 1.5 mMm MgCl2, 0.5nM primers, 10mMdNTPs, 2units Taq, 1010 copies of template b. none of these answers c. 1x buffer, 1.5 mMm MgCl2, 0.5pM primers, 10mMdNTPs, 2units Taq, 105 copies of template d. 1x buffer, 1.5 mMm NH3 Cl2, 0.5pM primers, 10mMdNTPs, 2units Taq, 102 copies of template e. 1x buffer, 1.5 mMm KCL2 , 0.5nM primers, 10mMdNTPs, 2units Taq, 103copies of template thank you!!arrow_forwardName four reagents used during a PCR reactionarrow_forward
- What would be the final primer concentration if 0.5 μl of 10 μM primers were added to a PCR reaction with a final volume of 20 μl?arrow_forwardWhat determines the size (length) of the primary PCR product? What might a successful gel check of a PCR reaction look like?arrow_forwardYou have added 4 μl of a 5 μM stock primer to a 25 μl PCR reaction. What is the amountof primer in nanomol that you have added to the reaction?arrow_forward
- In a 25 uL reaction, you desire a buffer concentration of 1X. You will be supplied with a stock solution of PCR buffer having a concentration of 10X. To a single 25 uL, how much 10X PCR buffer should be added to give a final concentration of 1X PCR buffer?arrow_forwardwhy in PCR we use different temperature ranges in each steps?arrow_forwardWhy is it possible to visualize a PCR product on an agarose gel even if the template genome is present at such a low concentration that it cannot be seen?arrow_forward
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