Essentials Of Genetics (9th Global Edition)
Essentials Of Genetics (9th Global Edition)
9th Edition
ISBN: 9780134143637
Author: William S. Klug, Michael R. Cummings
Publisher: Pearson Global Edition
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Chapter 17, Problem 2PDQ
Summary Introduction

To review:

The role of recombinant DNA technology (RDT) in genetic studies.

Introduction:

RDT is a technique that is used for creating recombinant DNA (deoxyribonucleic acid) molecule containing DNA from two different sources. RDT involves digestion of a vector molecule with a restriction enzyme (called molecular scissor) that cuts the DNA at their specific sites called restriction sites. Enzymes that cut DNA molecule are known as restriction enzymes. The two DNA then mixed and joined with the help of DNA ligase by forming a phosphodiester bond between two adjacent nucleotides. RDT is used for crop improvement, increasing protein quantity, and creating genomic and cDNA libraries. RDT allows studying the gene expression in an organism, which is independent of nonexpressed DNA.

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