Essentials Of Genetics (9th Global Edition)
9th Edition
ISBN: 9780134143637
Author: William S. Klug, Michael R. Cummings
Publisher: Pearson Global Edition
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Textbook Question
Chapter 17, Problem 3PDQ
What roles do restriction enzymes, vectors, and host cells play in recombinant DNA studies? What role does DNA ligase perform in a DNA cloning experiment? How does the action of DNA ligase differ from the function of restriction enzymes?
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What roles do restriction enzymes, vectors, and host cells play in recombinant DNA studies? What role does DNA ligase perform in a DNA cloning experiment? How does the action of DNA ligase differ from the function of restriction enzymes?
What ingredients are used in PCR? What role does each ingredient have in replicating DNA?
In making recombinant DNA, what is the benefit of using a restriction enzyme that cuts DNA in a staggered fashion?
Chapter 17 Solutions
Essentials Of Genetics (9th Global Edition)
Ch. 17 -
CASE STUDY |Should we worry about recombinant DNA...Ch. 17 - Prob. 2CSCh. 17 - Prob. 3CSCh. 17 -
HOW DO WE KNOW?
1. In this chapter we focused on...Ch. 17 - Prob. 2PDQCh. 17 - What roles do restriction enzymes, vectors, and...Ch. 17 - Prob. 4PDQCh. 17 - Prob. 5PDQCh. 17 - Prob. 6PDQCh. 17 - Prob. 7PDQ
Ch. 17 - List the advantages and disadvantages of using...Ch. 17 - What are the advantages of using a restriction...Ch. 17 - The introduction of genes into plants is a common...Ch. 17 - Prob. 11PDQCh. 17 - Prob. 12PDQCh. 17 - Prob. 13PDQCh. 17 - What advantages do cDNA libraries provide over...Ch. 17 - Prob. 15PDQCh. 17 -
16. List the steps involved in screening a...Ch. 17 -
17. In a typical PCR reaction, describe what is...Ch. 17 -
18. We usually think of enzymes as being most...Ch. 17 - How are dideoxynucleotides (ddNTPs) structurally...Ch. 17 - Prob. 20PDQCh. 17 - One complication of making a transgenic animal is...Ch. 17 -
22. When disrupting a mouse gene by knockout, why...Ch. 17 - Prob. 23PDQ
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- Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forwardWhich restriction enzyme used in your simulated electrophoresis experiment produced DNA with ‘sticky ends’? Which produced blunt ends? Of these two restriction enzymes, which would you choose to use as donor DNA to graft (or splice) onto a recipient strand of DNA, and why?arrow_forwardDuring PCR amplification in preparation for DNA sequencing, why were there different colors at the 3’ ends of the fragments produced? (What did these four colors represent?)arrow_forward
- Restriction enzymes and DNA ligase play essential roles in DNA cloning. How is it that a bacterium that produces a restriction enzyme does not cut its own DNA?arrow_forwardWhat is the role of streptomycin in CRISPR experiment? What biochemical changes (DNA, protein) occurred in those cells in which CRISPR worked?arrow_forwardWhat properties of DNA does PCR take advantage of?arrow_forward
- Restriction enzymes look for palindromic sequences of DNA to cut, how does it recognize those sequences? Does it bind to them and read their strands? Does it work with both strands of the DNA or does it just need one strand to work its process?arrow_forwardHow can we use software to identify restriction-enzyme cutting sites in sequenced DNA ?arrow_forwardWhat are restrictionenzymes? How do theseenzymes participate in therecombinant DNA technology?arrow_forward
- What sequence do the restriction enzymes used in the lab recognize? How do they cut? And how would these different cuts effect cloning? (i.e. compare and contrast how overhang and blunt cuts will differ with cloning.) What organisms were the restriction enzymes used in this lab derived from? Why are restriction enzymes important for the organism that makes them? What is the purpose of multiple cloning sites on pUC19? What size were each of your plasmid fragments?arrow_forwardWhat is a restriction enzyme? How can restriction enzymes be used to splice a piece of human DNA into a plasmid?arrow_forwardWhat are the disadvantages of doing DNA sequencing?arrow_forward
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