Essentials Of Genetics (9th Global Edition)
9th Edition
ISBN: 9780134143637
Author: William S. Klug, Michael R. Cummings
Publisher: Pearson Global Edition
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Textbook Question
Chapter 17, Problem 9PDQ
What are the advantages of using a restriction enzyme whose recognition site is relatively rare? When would you use such enzymes?
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What are the advantages of using a restriction enzyme whose recognitionsite is relatively rare? When would you use such enzymes?
What feature is commonly seen in the sequences recognized by type II restriction enzymes?
What does it mean if a restriction enzyme produces ‘sticky’ or ‘blunt’ ends? Does the restriction enzyme HaeIII produce “sticky” ends or “blunt” ends?
Chapter 17 Solutions
Essentials Of Genetics (9th Global Edition)
Ch. 17 -
CASE STUDY |Should we worry about recombinant DNA...Ch. 17 - Prob. 2CSCh. 17 - Prob. 3CSCh. 17 -
HOW DO WE KNOW?
1. In this chapter we focused on...Ch. 17 - Prob. 2PDQCh. 17 - What roles do restriction enzymes, vectors, and...Ch. 17 - Prob. 4PDQCh. 17 - Prob. 5PDQCh. 17 - Prob. 6PDQCh. 17 - Prob. 7PDQ
Ch. 17 - List the advantages and disadvantages of using...Ch. 17 - What are the advantages of using a restriction...Ch. 17 - The introduction of genes into plants is a common...Ch. 17 - Prob. 11PDQCh. 17 - Prob. 12PDQCh. 17 - Prob. 13PDQCh. 17 - What advantages do cDNA libraries provide over...Ch. 17 - Prob. 15PDQCh. 17 -
16. List the steps involved in screening a...Ch. 17 -
17. In a typical PCR reaction, describe what is...Ch. 17 -
18. We usually think of enzymes as being most...Ch. 17 - How are dideoxynucleotides (ddNTPs) structurally...Ch. 17 - Prob. 20PDQCh. 17 - One complication of making a transgenic animal is...Ch. 17 -
22. When disrupting a mouse gene by knockout, why...Ch. 17 - Prob. 23PDQ
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- For a restriction enzyme that recognizes the restriction site GGCC, Which of the following statements is/are true?arrow_forwardDescribe two features that are characteristic of the action of restriction endonucleases. How do these features make restriction endonucleases useful to genetic engineers?arrow_forwardWhat is a restriction enzyme? What structure does it recognize?What type of chemical bond does it cleave? Be as specific aspossible.arrow_forward
- If restriction endonucleases are produced by bacteria within a host, why don’t these enzymes chew up the genomic DNA of their host? What is the role of DNA methyltransferase in this?arrow_forwardIf restriction endonucleases are produced by bacteria within a host, why don’t these enzymes chew up the genomic DNA of their host? What is the role of DNA methyltransferase in this? Indicate the answerarrow_forwardWhy do bacteria make restriction endonucleases? What is it about the endonucleases that prevents bacteria from destroying their own DNA?arrow_forward
- If the following is a restriction enzyme: Sma I, A. What is the first letter represent? B. What do the next two letters represent? C. What does the roman numeral represent?arrow_forwardUsing the restriction site for SalI, suggest another restriction enzyme that would cut at the same site.arrow_forwardWhat would happen if the restriction enzymes do not cut the DNA at specific recognition sequences?arrow_forward
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