Essentials Of Genetics (9th Global Edition)
9th Edition
ISBN: 9780134143637
Author: William S. Klug, Michael R. Cummings
Publisher: Pearson Global Edition
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Chapter 17, Problem 12PDQ
Summary Introduction
To review:
The copy number of DNA (deoxyribonucleic acid) molecule after 15 polymerase chain reaction (PCR) cycles when the experiment started with only one copy of double-stranded DNA.
Introduction:
PCR is a laboratory techniquethatis used in molecular biologyexperiments. PCR involvesamplification of a DNA molecule, in order to generate millions of copies of a particular DNA segment.
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Assuming you started with only one copy of a DNA sequence, how many copies should be present after 15 cycles of PCR?
. In the PCR process, if we assume that each cycle takes5 minutes, how manyfold amplification would be accomplished in 1 hour?
During DNA isolation, you incubated the tissue sample at 55°C for 60 minutes, followed by incubation at 95°C for 10 minutes. What happens to the DNA molecules in this last 95°C incubation? What would happen to the PCR if this step was omitted?
Chapter 17 Solutions
Essentials Of Genetics (9th Global Edition)
Ch. 17 -
CASE STUDY |Should we worry about recombinant DNA...Ch. 17 - Prob. 2CSCh. 17 - Prob. 3CSCh. 17 -
HOW DO WE KNOW?
1. In this chapter we focused on...Ch. 17 - Prob. 2PDQCh. 17 - What roles do restriction enzymes, vectors, and...Ch. 17 - Prob. 4PDQCh. 17 - Prob. 5PDQCh. 17 - Prob. 6PDQCh. 17 - Prob. 7PDQ
Ch. 17 - List the advantages and disadvantages of using...Ch. 17 - What are the advantages of using a restriction...Ch. 17 - The introduction of genes into plants is a common...Ch. 17 - Prob. 11PDQCh. 17 - Prob. 12PDQCh. 17 - Prob. 13PDQCh. 17 - What advantages do cDNA libraries provide over...Ch. 17 - Prob. 15PDQCh. 17 -
16. List the steps involved in screening a...Ch. 17 -
17. In a typical PCR reaction, describe what is...Ch. 17 -
18. We usually think of enzymes as being most...Ch. 17 - How are dideoxynucleotides (ddNTPs) structurally...Ch. 17 - Prob. 20PDQCh. 17 - One complication of making a transgenic animal is...Ch. 17 -
22. When disrupting a mouse gene by knockout, why...Ch. 17 - Prob. 23PDQ
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- How many DNA molecules would exist after 30 PCR cycles?arrow_forwardAfter one round of PCR, one molecule of DNA consisting of two complementary strands yields how many molecules of DNA for a total of how many strands?arrow_forwardHow many copies of a target DNA molecule are made after 30 cycles of PCR?arrow_forward
- What is expected theoretical number of copies of DNA molecules after 28 cycles in a PCR experiment? What is the percent efficiency of the PCR experiment if the actual number of copies was 500,000,000? Show your calculationsarrow_forwardIf you wanted to prepare only one PCR reaction, how much of each reagent would you add to the PCR tube?arrow_forwardIf you performed PCR to amplify a gene region, and you began with 20 copies of double stranded genome, after 18 cycles, about how many double stranded copies of that region would you have (in theory)?arrow_forward
- After four cycles of PCR, which products predominate? Explain why.arrow_forwardHow is DNA amplified in a polymerase chain reaction(PCR) procedure?arrow_forwardA scientist programed his PCR machine to perform five complete cycles of PCR starting with one double stranded DNA molecule, how many molecules of DNA did he get at the end assuming all conditions went normally?arrow_forward
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