Essentials Of Genetics (9th Global Edition)
Essentials Of Genetics (9th Global Edition)
9th Edition
ISBN: 9780134143637
Author: William S. Klug, Michael R. Cummings
Publisher: Pearson Global Edition
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Chapter 17, Problem 12PDQ
Summary Introduction

To review:

The copy number of DNA (deoxyribonucleic acid) molecule after 15 polymerase chain reaction (PCR) cycles when the experiment started with only one copy of double-stranded DNA.

Introduction:

PCR is a laboratory techniquethatis used in molecular biologyexperiments. PCR involvesamplification of a DNA molecule, in order to generate millions of copies of a particular DNA segment.

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Assuming you started with only one copy of a DNA sequence, how many copies should be present after 15 cycles of PCR?
. In the PCR process, if we assume that each cycle takes5 minutes, how manyfold amplification would be accomplished in 1 hour?
During DNA isolation, you incubated the tissue sample at 55°C for 60 minutes, followed by incubation at 95°C for 10 minutes.   What happens to the DNA molecules in this last 95°C incubation?  What would happen to the PCR if this step was omitted?
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Molecular Techniques: Basic Concepts; Author: Dr. A's Clinical Lab Videos;https://www.youtube.com/watch?v=7HFHZy8h6z0;License: Standard Youtube License