Genetics: Analysis and Principles
Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 24, Problem 12EQ
Summary Introduction

To review:

A sequence of elements with two examples and the process of determination ofsequence elements by a computer program or by genetic studies.

Introduction:

Deoxyribonucleic acid (DNA) contains the genetic information about the organism and its sequencing is done to identify the order of each nucleotide in a genome. DNA sequencing is done in order to identify the functions and pathways of protein formation.

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The goal of many computer programs is to identify sequenceelements within a long segment of DNA. What is a sequenceelement? Give two examples. How is the specific sequence of asequence element determined? In other words, is it determined bythe computer program or by experimentation? Explain.
The goal of many computer programs is to identify sequence elementswithin a long segment of DNA. What is a sequence element? Givetwo examples. How is the specific sequence of a sequence elementdetermined? In other words, is it determined by the computer programor by genetic studies? Explain.
What does the symbol “N” indicate (see the arrow)? Is this a problem for getting an accurate DNA sequence?  The height of the peak in the figure is an indication of the quality of the data, with a high peak correlated with a low error in “base-calling” of perhaps 1 error in 10,000 bases. Is this generally a high-quality DNA sequence for determining the true DNA sequence of the gene studied? How can you tell? Which end of the sequence shown above is the 5’ end of the sequence being made via PCR.
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