Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 24, Problem 1EQ
Summary Introduction
To review:
A. The substance attached to the slide, particularly about the number of spots, length of DNA (deoxyribonucleic acid) fragments and origin of DNA fragments.
B. The substance hybridized to the microarray.
C. The process of detecting hybridization.
Introduction:
A DNA microarray contains a compilation of numerous microscopic spots of DNA such as, DNA chip, gene chip and gene array which are attached to the solid surface. It is used to study gene expression levels and are employed as diagnostic tests for detecting diseases.
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Chapter 24 Solutions
Genetics: Analysis and Principles
Ch. 24.1 - 1. A DNA microarray is a slide that is dotted...Ch. 24.1 - 2. The purpose of a ChIP-chip assay is to...Ch. 24.1 - 3. For the method of RNA sequencing (RNA-Seq),...Ch. 24.1 - A gene knockout is a gene a. whose function has...Ch. 24.2 - Prob. 1COMQCh. 24.2 - Prob. 2COMQCh. 24.2 - Prob. 3COMQCh. 24.2 - Prob. 4COMQCh. 24.3 - Prob. 1COMQCh. 24.3 - 2. Homologous genes
a. are derived from the same...
Ch. 24.3 - Prob. 3COMQCh. 24 - 1. Give the meanings of the following terms:...Ch. 24 - Prob. 2CONQCh. 24 - What is a database? What types of information are...Ch. 24 - Prob. 4CONQCh. 24 - Prob. 5CONQCh. 24 - Prob. 6CONQCh. 24 - Prob. 7CONQCh. 24 - Prob. 8CONQCh. 24 - Prob. 1EQCh. 24 - In the procedure called RNA sequencing (RNA-Seq),...Ch. 24 - 3. Can two-dimensional gel electrophoresis be used...Ch. 24 - Prob. 4EQCh. 24 - 5. Describe the two general types of protein...Ch. 24 - 6. Discuss the bioinformatics approaches that can...Ch. 24 - 7. What is a motif? Why is it useful for computer...Ch. 24 - Discuss why it is useful to search a database to...Ch. 24 - Prob. 9EQCh. 24 - In this chapter, we considered a computer program...Ch. 24 - Prob. 11EQCh. 24 - Prob. 12EQCh. 24 - Prob. 13EQCh. 24 - Refer to question 3 in More Genetic TIPS before...Ch. 24 - Prob. 15EQCh. 24 - Prob. 16EQCh. 24 - 1. Let’s suppose you are in charge of organizing...
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- What information can be gathered by using DNA gel electrophoresis? Describe the steps in the process and explain how and why gel electrophoresis can be used to separate DNA fragments.arrow_forwardDuring PCR amplification in preparation for DNA sequencing, why were there different colors at the 3’ ends of the fragments produced? (What did these four colors represent?)arrow_forwardAbove are the results of gel electrophoresis following digestion with restriction enzymes. What is the total length of the DNA fragment? Which enzyme, HindIII or EcoRI, produced a larger fragment? What part of DNA causes it to be negatively charged in order for electrophoresis to work?arrow_forward
- If you were to isolate genomic DNA from two people and perform restriction enzyme digestion followed by agarose gel electrophoresis, would this be an effective way to distinguish one person from the other? Explain. If not, what is another technique you might use to distinguish the two people?arrow_forwardWhat are the steps in extracting DNA from a Banana using simple household materials like dishwashing liquid and cold isopropyl alcohol? Explain the principlesarrow_forwardWhat are STRs, and why are they useful for DNA profiling?arrow_forward
- How does gel electrophoresis separate DNA fragments of your unknown sample and How will you be able to track the distance travelled by the DNA during electrophoretic run?arrow_forwardYou have isolated genomic DNA and plasmid DNA from a bacterial culture. Please answer the following questions: 1. What solution did you use to elute DNA from the silica resin column? 2. What is the function of the chaotropic salt in DNA isolation? 3. Why do we add the chaotropic salt for genomic DNA before lysis and for plasmid isolation we add the salts after the lysis step?arrow_forwardHow are DNA molecules visualized in a gel after electrophoresis? Why do DNA molecules migrate toward the + electrode? What determines the rate of their migration? What is the effect of PEG on DNA fragments of different sizes? How is this influenced by the concentration of PEG?arrow_forward
- How does what you see on the acrylamide gel reflect what is presented on a sequencing chromatograph?arrow_forwardIf it is not possible to have three bands for one STR region when looking at the DNA from one individual, then how many different STR regions were sampled in the gel electrophoresis simulation to end up with the three bands on the gel. Note: there are several possible correct answers to this question.arrow_forwardThe following question is related to Restriction Enzymes and RFLP. Using EcoRl, the smallest fragments between DNA sequence A and B were how many base pairs?arrow_forward
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