Concept explainers
(a)
To identify: The first residue obtained by Edman degradation of cytochrome c from Drosophila.
Concept introduction: Edman degradation is the process for sequencing amino acids in a peptide. In this technique, the N-terminal of the amino acid is labeled and cleaved from the peptide chain without disturbing the peptide linkages between other amino acid residues. This is used to identify the protein and post-translational modifications in the sequence.
(b)
To identify: The first residue obtained by Edman degradation of cytochrome c from baker’s
yeast.
Concept introduction: Edman degradation is the process for sequencing amino acids in a peptide. In this technique, the N-terminal of the amino acid is labeled and cleaved from the peptide chain without disturbing the peptide linkages between other amino acid residues. This is used to identify the protein and post-translational modifications in the sequence.
(c)
To identify: The first residue obtained by Edman degradation of cytochrome c from wheat germ.
Concept introduction: Edman degradation is the process for sequencing amino acids in a peptide. In this technique, the N-terminal of amino acid is labeled and cleaved from the peptide chain without disturbing the peptide linkages between other amino acid residues. This is used to identify the protein and post-translational modifications in the sequence.
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