The position of disulfide bonds in the intact polypeptide. Concept introduction: Proteins are made up of one or more amino acids linked together to form a chain. The building blocks of the protein is called amino acid. Amino acids are termed as residues if they release a hydrogen ion from the amine end or a hydroxyl ion from the carboxyl end, or both; a water molecule is released as a result of formation of each amide bond. When more than eight peptides are linked together by peptide bonds, it forms a polypeptide. Given: Treatment of a polypeptide with 2-mercaptoethanol yields two polypeptides: Ala-Val-Cys-Arg-Thr-Gly-Cys-Lys-Asn-Phe-Leu, Tyr-Lys-Cys-Phe-Arg-His-Thr-Lys-Cys-Ser. Treatment of the intact polypeptide with trypsin yields seven peptide fragments: Ala, Arg, Cys 2 , Ser, Val; Arg, Cys 2 , Gly, Lys, Thr, Phe; Asn, Leu, Phe; His, Lys, Thr; Lys, Tyr.

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Answer 1

Question

Chapter 5, Problem 33CQ

Summary Introduction

To determine: The position of disulfide bonds in the intact polypeptide.

Concept introduction: Proteins are made up of one or more amino acids linked together to form a chain. The building blocks of the protein is called amino acid. Amino acids are termed as residues if they release a hydrogen ion from the amine end or a hydroxyl ion from the carboxyl end, or both; a water molecule is released as a result of formation of each amide bond. When more than eight peptides are linked together by peptide bonds, it forms a polypeptide.

Given:

Treatment of a polypeptide with 2-mercaptoethanol yields two polypeptides: Ala-Val-Cys-Arg-Thr-Gly-Cys-Lys-Asn-Phe-Leu, Tyr-Lys-Cys-Phe-Arg-His-Thr-Lys-Cys-Ser.

Treatment of the intact polypeptide with trypsin yields seven peptide fragments: Ala, Arg, Cys₂, Ser, Val; Arg, Cys₂, Gly, Lys, Thr, Phe; Asn, Leu, Phe; His, Lys, Thr; Lys, Tyr.

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Answer 2

Question

Chapter 5, Problem 33CQ

Summary Introduction

To determine: The position of disulfide bonds in the intact polypeptide.

Concept introduction: Proteins are made up of one or more amino acids linked together to form a chain. The building blocks of the protein is called amino acid. Amino acids are termed as residues if they release a hydrogen ion from the amine end or a hydroxyl ion from the carboxyl end, or both; a water molecule is released as a result of formation of each amide bond. When more than eight peptides are linked together by peptide bonds, it forms a polypeptide.

Given:

Treatment of a polypeptide with 2-mercaptoethanol yields two polypeptides: Ala-Val-Cys-Arg-Thr-Gly-Cys-Lys-Asn-Phe-Leu, Tyr-Lys-Cys-Phe-Arg-His-Thr-Lys-Cys-Ser.

Treatment of the intact polypeptide with trypsin yields seven peptide fragments: Ala, Arg, Cys₂, Ser, Val; Arg, Cys₂, Gly, Lys, Thr, Phe; Asn, Leu, Phe; His, Lys, Thr; Lys, Tyr.

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Answer 3

Question

Chapter 5, Problem 33CQ

Summary Introduction

To determine: The position of disulfide bonds in the intact polypeptide.

Concept introduction: Proteins are made up of one or more amino acids linked together to form a chain. The building blocks of the protein is called amino acid. Amino acids are termed as residues if they release a hydrogen ion from the amine end or a hydroxyl ion from the carboxyl end, or both; a water molecule is released as a result of formation of each amide bond. When more than eight peptides are linked together by peptide bonds, it forms a polypeptide.

Given:

Treatment of a polypeptide with 2-mercaptoethanol yields two polypeptides: Ala-Val-Cys-Arg-Thr-Gly-Cys-Lys-Asn-Phe-Leu, Tyr-Lys-Cys-Phe-Arg-His-Thr-Lys-Cys-Ser.

Treatment of the intact polypeptide with trypsin yields seven peptide fragments: Ala, Arg, Cys₂, Ser, Val; Arg, Cys₂, Gly, Lys, Thr, Phe; Asn, Leu, Phe; His, Lys, Thr; Lys, Tyr.

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Answer 4

Question

Chapter 5, Problem 33CQ

Summary Introduction

To determine: The position of disulfide bonds in the intact polypeptide.

Concept introduction: Proteins are made up of one or more amino acids linked together to form a chain. The building blocks of the protein is called amino acid. Amino acids are termed as residues if they release a hydrogen ion from the amine end or a hydroxyl ion from the carboxyl end, or both; a water molecule is released as a result of formation of each amide bond. When more than eight peptides are linked together by peptide bonds, it forms a polypeptide.

Given:

Treatment of a polypeptide with 2-mercaptoethanol yields two polypeptides: Ala-Val-Cys-Arg-Thr-Gly-Cys-Lys-Asn-Phe-Leu, Tyr-Lys-Cys-Phe-Arg-His-Thr-Lys-Cys-Ser.

Treatment of the intact polypeptide with trypsin yields seven peptide fragments: Ala, Arg, Cys₂, Ser, Val; Arg, Cys₂, Gly, Lys, Thr, Phe; Asn, Leu, Phe; His, Lys, Thr; Lys, Tyr.

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Answer 5

Chapter 5, Problem 33CQ

Summary Introduction

To determine: The position of disulfide bonds in the intact polypeptide.

Concept introduction: Proteins are made up of one or more amino acids linked together to form a chain. The building blocks of the protein is called amino acid. Amino acids are termed as residues if they release a hydrogen ion from the amine end or a hydroxyl ion from the carboxyl end, or both; a water molecule is released as a result of formation of each amide bond. When more than eight peptides are linked together by peptide bonds, it forms a polypeptide.

Given:

Treatment of a polypeptide with 2-mercaptoethanol yields two polypeptides: Ala-Val-Cys-Arg-Thr-Gly-Cys-Lys-Asn-Phe-Leu, Tyr-Lys-Cys-Phe-Arg-His-Thr-Lys-Cys-Ser.

Treatment of the intact polypeptide with trypsin yields seven peptide fragments: Ala, Arg, Cys₂, Ser, Val; Arg, Cys₂, Gly, Lys, Thr, Phe; Asn, Leu, Phe; His, Lys, Thr; Lys, Tyr.

Answer 6

Chapter 5, Problem 33CQ

Summary Introduction

To determine: The position of disulfide bonds in the intact polypeptide.

Concept introduction: Proteins are made up of one or more amino acids linked together to form a chain. The building blocks of the protein is called amino acid. Amino acids are termed as residues if they release a hydrogen ion from the amine end or a hydroxyl ion from the carboxyl end, or both; a water molecule is released as a result of formation of each amide bond. When more than eight peptides are linked together by peptide bonds, it forms a polypeptide.

Given:

Treatment of a polypeptide with 2-mercaptoethanol yields two polypeptides: Ala-Val-Cys-Arg-Thr-Gly-Cys-Lys-Asn-Phe-Leu, Tyr-Lys-Cys-Phe-Arg-His-Thr-Lys-Cys-Ser.

Treatment of the intact polypeptide with trypsin yields seven peptide fragments: Ala, Arg, Cys₂, Ser, Val; Arg, Cys₂, Gly, Lys, Thr, Phe; Asn, Leu, Phe; His, Lys, Thr; Lys, Tyr.

Answer 7

Chapter 5, Problem 33CQ

Summary Introduction

To determine: The position of disulfide bonds in the intact polypeptide.

Concept introduction: Proteins are made up of one or more amino acids linked together to form a chain. The building blocks of the protein is called amino acid. Amino acids are termed as residues if they release a hydrogen ion from the amine end or a hydroxyl ion from the carboxyl end, or both; a water molecule is released as a result of formation of each amide bond. When more than eight peptides are linked together by peptide bonds, it forms a polypeptide.

Given:

Treatment of a polypeptide with 2-mercaptoethanol yields two polypeptides: Ala-Val-Cys-Arg-Thr-Gly-Cys-Lys-Asn-Phe-Leu, Tyr-Lys-Cys-Phe-Arg-His-Thr-Lys-Cys-Ser.

Treatment of the intact polypeptide with trypsin yields seven peptide fragments: Ala, Arg, Cys₂, Ser, Val; Arg, Cys₂, Gly, Lys, Thr, Phe; Asn, Leu, Phe; His, Lys, Thr; Lys, Tyr.

Answer 8

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Answer 9

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Answer 10

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Answer 11

Ch. 5 - Prob. 1E Ch. 5 - Prob. 2E Ch. 5 - 3. Which peptide has greater absorbance at 280...Ch. 5 - 4. Protein X has an absorptivity of 0.4 mL · mg?1...Ch. 5 - 5. You are using ammonium sulfate to purify...Ch. 5 - Prob. 6E Ch. 5 - 7. (a) In what order would the amino acids Arg,...Ch. 5 - Prob. 8E Ch. 5 - 9. Explain why a certain protein has an apparent...Ch. 5 - 10. Determine the subunit composition of a protein...

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