Concept explainers
(a)
To explain: The reason for the different positive charges on different particles of the protein.
Concept introduction: The proteins involves creating positively charged ions of the protein and separating them, according to their mass to charge ratio (m/z) is called Electrospray ionization mass spectrometry (ESI-MS). It is the ionization technique to produce ions using an electrospray from macromolecules.
(b)
To determine: The maximum positive charge present in hen egg-white lysozyme (HEWL).
Concept introduction: The proteins involves creating positively charged ions of the protein and separating them, according to their mass to charge ratio (m/z) is called Electrospray ionization mass spectrometry (ESI-MS). It is the ionization technique to produce ions using an electrospray from macromolecules.
Given: The amino acid composition (in numbers of residues per chain) of hen egg-white lysozyme (HEWL) is as follows:
P 2 Y 3 N 14 H 1
D 7 M 2 L 8 E 2
C 8 R 11 G 12 F 3
A 12 I 6 K 6 V 6
S 10 W 6 T 7 Q 3
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