The ways to link the two polypeptide chains of protein through the disulfide bonds that was denatured by mercaptoethanol. Concept introduction: Disulfide bonds play a vital role in folding and maintaining the stability of proteins. To explore the protein structure, it is important to break the disulfide bond. Mercaptoethanol is the chemical compound mainly used to reduce the disulfide bonds in proteins and to protect the sulfhydryl groups from oxidation. There are two side chains in the mercaptoethanol with the molecular weight of 40kD and 60kD, respectively.

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Answer 1

Question

Chapter 5, Problem 2E

Summary Introduction

To explain: The ways to link the two polypeptide chains of protein through the disulfide bonds that was denatured by mercaptoethanol.

Concept introduction: Disulfide bonds play a vital role in folding and maintaining the stability of proteins. To explore the protein structure, it is important to break the disulfide bond. Mercaptoethanol is the chemical compound mainly used to reduce the disulfide bonds in proteins and to protect the sulfhydryl groups from oxidation. There are two side chains in the mercaptoethanol with the molecular weight of 40kD and 60kD, respectively.

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Answer 2

Question

Chapter 5, Problem 2E

Summary Introduction

To explain: The ways to link the two polypeptide chains of protein through the disulfide bonds that was denatured by mercaptoethanol.

Concept introduction: Disulfide bonds play a vital role in folding and maintaining the stability of proteins. To explore the protein structure, it is important to break the disulfide bond. Mercaptoethanol is the chemical compound mainly used to reduce the disulfide bonds in proteins and to protect the sulfhydryl groups from oxidation. There are two side chains in the mercaptoethanol with the molecular weight of 40kD and 60kD, respectively.

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Answer 3

Question

Chapter 5, Problem 2E

Summary Introduction

To explain: The ways to link the two polypeptide chains of protein through the disulfide bonds that was denatured by mercaptoethanol.

Concept introduction: Disulfide bonds play a vital role in folding and maintaining the stability of proteins. To explore the protein structure, it is important to break the disulfide bond. Mercaptoethanol is the chemical compound mainly used to reduce the disulfide bonds in proteins and to protect the sulfhydryl groups from oxidation. There are two side chains in the mercaptoethanol with the molecular weight of 40kD and 60kD, respectively.

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Answer 4

Question

Chapter 5, Problem 2E

Summary Introduction

To explain: The ways to link the two polypeptide chains of protein through the disulfide bonds that was denatured by mercaptoethanol.

Concept introduction: Disulfide bonds play a vital role in folding and maintaining the stability of proteins. To explore the protein structure, it is important to break the disulfide bond. Mercaptoethanol is the chemical compound mainly used to reduce the disulfide bonds in proteins and to protect the sulfhydryl groups from oxidation. There are two side chains in the mercaptoethanol with the molecular weight of 40kD and 60kD, respectively.

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Answer 5

Chapter 5, Problem 2E

Summary Introduction

To explain: The ways to link the two polypeptide chains of protein through the disulfide bonds that was denatured by mercaptoethanol.

Concept introduction: Disulfide bonds play a vital role in folding and maintaining the stability of proteins. To explore the protein structure, it is important to break the disulfide bond. Mercaptoethanol is the chemical compound mainly used to reduce the disulfide bonds in proteins and to protect the sulfhydryl groups from oxidation. There are two side chains in the mercaptoethanol with the molecular weight of 40kD and 60kD, respectively.

Answer 6

Chapter 5, Problem 2E

Summary Introduction

To explain: The ways to link the two polypeptide chains of protein through the disulfide bonds that was denatured by mercaptoethanol.

Concept introduction: Disulfide bonds play a vital role in folding and maintaining the stability of proteins. To explore the protein structure, it is important to break the disulfide bond. Mercaptoethanol is the chemical compound mainly used to reduce the disulfide bonds in proteins and to protect the sulfhydryl groups from oxidation. There are two side chains in the mercaptoethanol with the molecular weight of 40kD and 60kD, respectively.

Answer 7

Chapter 5, Problem 2E

Summary Introduction

To explain: The ways to link the two polypeptide chains of protein through the disulfide bonds that was denatured by mercaptoethanol.

Concept introduction: Disulfide bonds play a vital role in folding and maintaining the stability of proteins. To explore the protein structure, it is important to break the disulfide bond. Mercaptoethanol is the chemical compound mainly used to reduce the disulfide bonds in proteins and to protect the sulfhydryl groups from oxidation. There are two side chains in the mercaptoethanol with the molecular weight of 40kD and 60kD, respectively.

Answer 8

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Answer 9

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Answer 10

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Answer 11

Ch. 5 - Prob. 1E Ch. 5 - Prob. 2E Ch. 5 - 3. Which peptide has greater absorbance at 280...Ch. 5 - 4. Protein X has an absorptivity of 0.4 mL · mg?1...Ch. 5 - 5. You are using ammonium sulfate to purify...Ch. 5 - Prob. 6E Ch. 5 - 7. (a) In what order would the amino acids Arg,...Ch. 5 - Prob. 8E Ch. 5 - 9. Explain why a certain protein has an apparent...Ch. 5 - 10. Determine the subunit composition of a protein...

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