Concept explainers
(a)
To explain: The order of amino acids Arg, His, and Leu that eluted from carboxymethyl column at pH 6.
Concept introduction: Separation and purification of biological macromolecules are done using carboxymethyl columns. These columns have carboxymethyl functional groups –CH2OCH2COOH. At neutral pH, the carboxymethyl group is ionized and becomes negatively charged. Hence, it is a weak cation exchanger.
(b)
To explain: The order of amino acids Glu, Lys, and Val that are eluted from diethylaminoethyl column at pH 8.
Concept introduction: DEAE (Diethylaminoethyl) cellulose chromatography is an ion exchange chromatography used for the separation and purification of proteins. DEAE cellulose is a weak anion exchanger, so the negatively charged protein binds to DEAE. It separates the substance based on their charges, where a negatively charged protein binds with the positively charged DEAE.
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Chapter 5 Solutions
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