The sequence of the intact peptide. Concept introduction: Proteins are made up of one or more amino acids linked together to form a chain. The building blocks of the protein is called amino acid. Amino acids are termed as residues if they release a hydrogen ion from the amine end or a hydroxyl ion from the carboxyl end, or both; a water molecule is released as a result of formation of each amide bond. When more than eight peptides are linked together by peptide bonds, it forms a polypeptide. Given: Cleavage with cyanogen bromide (CNBr) yields three smaller peptides: Arg-Ala-Tyr-Gly-Asn, Leu-Phe-Met, Asp-Met. Cleavage with chymotrypsin yields three peptides: Met-Arg-Ala-Tyr, Asp-Met-Leu-Phe, Gly-Asn.

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Answer 1

Question

Chapter 5, Problem 32CQ

Summary Introduction

To determine: The sequence of the intact peptide.

Concept introduction: Proteins are made up of one or more amino acids linked together to form a chain. The building blocks of the protein is called amino acid. Amino acids are termed as residues if they release a hydrogen ion from the amine end or a hydroxyl ion from the carboxyl end, or both; a water molecule is released as a result of formation of each amide bond. When more than eight peptides are linked together by peptide bonds, it forms a polypeptide.

Given:

Cleavage with cyanogen bromide (CNBr) yields three smaller peptides: Arg-Ala-Tyr-Gly-Asn, Leu-Phe-Met, Asp-Met.

Cleavage with chymotrypsin yields three peptides: Met-Arg-Ala-Tyr, Asp-Met-Leu-Phe, Gly-Asn.

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Answer 2

Question

Chapter 5, Problem 32CQ

Summary Introduction

To determine: The sequence of the intact peptide.

Concept introduction: Proteins are made up of one or more amino acids linked together to form a chain. The building blocks of the protein is called amino acid. Amino acids are termed as residues if they release a hydrogen ion from the amine end or a hydroxyl ion from the carboxyl end, or both; a water molecule is released as a result of formation of each amide bond. When more than eight peptides are linked together by peptide bonds, it forms a polypeptide.

Given:

Cleavage with cyanogen bromide (CNBr) yields three smaller peptides: Arg-Ala-Tyr-Gly-Asn, Leu-Phe-Met, Asp-Met.

Cleavage with chymotrypsin yields three peptides: Met-Arg-Ala-Tyr, Asp-Met-Leu-Phe, Gly-Asn.

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Answer 3

Question

Chapter 5, Problem 32CQ

Summary Introduction

To determine: The sequence of the intact peptide.

Concept introduction: Proteins are made up of one or more amino acids linked together to form a chain. The building blocks of the protein is called amino acid. Amino acids are termed as residues if they release a hydrogen ion from the amine end or a hydroxyl ion from the carboxyl end, or both; a water molecule is released as a result of formation of each amide bond. When more than eight peptides are linked together by peptide bonds, it forms a polypeptide.

Given:

Cleavage with cyanogen bromide (CNBr) yields three smaller peptides: Arg-Ala-Tyr-Gly-Asn, Leu-Phe-Met, Asp-Met.

Cleavage with chymotrypsin yields three peptides: Met-Arg-Ala-Tyr, Asp-Met-Leu-Phe, Gly-Asn.

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Answer 4

Question

Chapter 5, Problem 32CQ

Summary Introduction

To determine: The sequence of the intact peptide.

Concept introduction: Proteins are made up of one or more amino acids linked together to form a chain. The building blocks of the protein is called amino acid. Amino acids are termed as residues if they release a hydrogen ion from the amine end or a hydroxyl ion from the carboxyl end, or both; a water molecule is released as a result of formation of each amide bond. When more than eight peptides are linked together by peptide bonds, it forms a polypeptide.

Given:

Cleavage with cyanogen bromide (CNBr) yields three smaller peptides: Arg-Ala-Tyr-Gly-Asn, Leu-Phe-Met, Asp-Met.

Cleavage with chymotrypsin yields three peptides: Met-Arg-Ala-Tyr, Asp-Met-Leu-Phe, Gly-Asn.

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Answer 5

Chapter 5, Problem 32CQ

Summary Introduction

To determine: The sequence of the intact peptide.

Concept introduction: Proteins are made up of one or more amino acids linked together to form a chain. The building blocks of the protein is called amino acid. Amino acids are termed as residues if they release a hydrogen ion from the amine end or a hydroxyl ion from the carboxyl end, or both; a water molecule is released as a result of formation of each amide bond. When more than eight peptides are linked together by peptide bonds, it forms a polypeptide.

Given:

Cleavage with cyanogen bromide (CNBr) yields three smaller peptides: Arg-Ala-Tyr-Gly-Asn, Leu-Phe-Met, Asp-Met.

Cleavage with chymotrypsin yields three peptides: Met-Arg-Ala-Tyr, Asp-Met-Leu-Phe, Gly-Asn.

Answer 6

Chapter 5, Problem 32CQ

Summary Introduction

To determine: The sequence of the intact peptide.

Concept introduction: Proteins are made up of one or more amino acids linked together to form a chain. The building blocks of the protein is called amino acid. Amino acids are termed as residues if they release a hydrogen ion from the amine end or a hydroxyl ion from the carboxyl end, or both; a water molecule is released as a result of formation of each amide bond. When more than eight peptides are linked together by peptide bonds, it forms a polypeptide.

Given:

Cleavage with cyanogen bromide (CNBr) yields three smaller peptides: Arg-Ala-Tyr-Gly-Asn, Leu-Phe-Met, Asp-Met.

Cleavage with chymotrypsin yields three peptides: Met-Arg-Ala-Tyr, Asp-Met-Leu-Phe, Gly-Asn.

Answer 7

Chapter 5, Problem 32CQ

Summary Introduction

To determine: The sequence of the intact peptide.

Concept introduction: Proteins are made up of one or more amino acids linked together to form a chain. The building blocks of the protein is called amino acid. Amino acids are termed as residues if they release a hydrogen ion from the amine end or a hydroxyl ion from the carboxyl end, or both; a water molecule is released as a result of formation of each amide bond. When more than eight peptides are linked together by peptide bonds, it forms a polypeptide.

Given:

Cleavage with cyanogen bromide (CNBr) yields three smaller peptides: Arg-Ala-Tyr-Gly-Asn, Leu-Phe-Met, Asp-Met.

Cleavage with chymotrypsin yields three peptides: Met-Arg-Ala-Tyr, Asp-Met-Leu-Phe, Gly-Asn.

Answer 8

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Answer 9

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Answer 10

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Answer 11

Ch. 5 - Prob. 1E Ch. 5 - Prob. 2E Ch. 5 - 3. Which peptide has greater absorbance at 280...Ch. 5 - 4. Protein X has an absorptivity of 0.4 mL · mg?1...Ch. 5 - 5. You are using ammonium sulfate to purify...Ch. 5 - Prob. 6E Ch. 5 - 7. (a) In what order would the amino acids Arg,...Ch. 5 - Prob. 8E Ch. 5 - 9. Explain why a certain protein has an apparent...Ch. 5 - 10. Determine the subunit composition of a protein...

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