Concept explainers
a.
To determine:
The proportions of PD, NPD and T tetrads that are expected to be present in yeast.
Introduction:
The genotype of an organism is dependent on its genome and also on the environment of the organism. In some of the animals, it is not as typical as the genotype of the mother plays a crucial role. This type of effect is known as a maternal effect. The genes that participate in this are called maternal effect genes.
b.
To determine:
The type and proportion of tetrads.
Introduction:
The genes are the sequence of
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Genetics: From Genes to Genomes, 5th edition
- Neurospora of genotype a + c are crossed withNeurospora of genotype + b +. (Here, + is shorthandfor the wild-type allele.) The following tetrads areobtained (note that the genotype of the four sporepairs in an ascus are listed, rather than listing alleight spores):a + c a b c + + c + b c a b + a + ca + c a b c a + c a b c a b + a b c+ b + + + + + b + + + + + + c + + ++ b + + + + a b + a + + + + c + b +137 141 26 25 2 3a. In how many cells has meiosis occurred to yieldthese data?b. Give the best genetic map to explain these results.Indicate all relevant genetic distances, both betweengenes and between each gene and the centromere.c. Diagram a meiosis that could give rise to oneof the three tetrads in the class at the far right inthe listarrow_forwardImagine that you have done a cross between two strains of yeast, one of which has the genotype A B C and the other a b c, where the letters refer to three closely linked genes in the order given. You examine many tetrads resulting from this cross, and the two tetrads below are found that have atypical scoring patterns. In tetrad I, the spores are A B C, ABC, a B c, and a b c. In tetrad II, the spores are A B C, A b c, a b C, and a b c. Which tetrad or tetrads indicate(s) that gene conversion has occurred AND show(s) that recombination has occurred between genes A and C? O neither tetrads I and II O tetrad II O tetrad I O both tetrads I and IIarrow_forwardAlleles of genes A and B were analyzed in Neurospora according to the cross shown below. Ordered tetrads are summarized in each horizontal row with the number of tetrads in each category listed alongside. A) Analyze the data to determine the recombination frequency (RF) between A and B, along with any additional information that is available from this data. B) Draw a map of the chromosome or chromosomes with appropriate map distances. C) Use the Perkins formula to reanalyze any relationship between A and B.arrow_forward
- EC2. Here is a tetrad produced by mating a H Y strain to an h y strain. a) What is the tetrad type? b) What has recombined with what? EC3. In corn, a dihybrid for the recessive genes a and b is test-crossed. The distribution of the phenotypes is as follows: A B 122A b 118a B 81a b 79 a) Do the genes appear to be sorting independently? Look at map units here. b) Test your hypothesis with a chi-squared test.arrow_forwardIn the fungus Neurospora, a strain that is auxotrophic for thiamine (mutant allele t) was crossed with a strain that isauxotrophic for methionine (mutant allele m). Linear asci were isolated and classified into the following groups: a. Determine the linkage relations of these two genes to their centromere(s) and to each other. Specify distances in map units. b. Draw a diagram to show the origin of the ascus type with only one single representative (second from right).arrow_forwardConsider the first category of test-cross offspring shown in figure 8.2 (+b, LS). Consider also that the parents of the heterozygous female flies in the test cross had the following genotypes: bb, SS, and +, LL. A. What would be the physical phenotype of these flies? B. If PC was conducted with the DNA of one of these flies using the primers for the molecular marker, what would be the appearance of the bands on an electrophoresis gel with the PC products? C. If the gene for black body and the locus for the molecular marker (L long or S short) were unlinked, what proportion of the test-cross progeny would be black flies that are heterozygous for the molecular marker? What proportion would be flies with normal body color, which are homozygous for one form of the molecular marker? D. If the gene for black body and the locus for the molecular marker were linked, how would the proportion of flies be different?arrow_forward
- In tomato the mutant genes o (oblate=flattened fruit), p (peach=hairy fruit) and s (compound inflorescence) were found to be in chromosome 2. The test cross results are: 73 110 + 348 2 2 + 306 p 96 63 a) Construct the linkage map b) What are the genotypes of the homozygous parents used in making the F1 heterozygote? c) Compute for the coefficient of coincidence. O o O O +arrow_forwardIndicate the percentage of tetrads that would have 0,1, 2, 3, or 4 viable spores after Saccharomycescerevisiae a / α diploids of the following genotypesare sporulated:a. A true-breeding wild-type strain (with no mutations in any gene essential for viability).b. A strain heterozygous for a null (completely inactivating) mutation in a single essential gene.For the remaining parts of this problem, considercrosses between yeast strains of the form a × b, wherea and b are both temperature-sensitive mutations indifferent essential genes. The cross is conducted under permissive (low-temperature) conditions. Indicatethe percentage of tetrads that would have 0, 1, 2, 3, or4 viable spores subsequently measured under restrictive (high-temperature) conditions.c. a and b are unlinked, and both are 0 m.u. from thecentromere.d. a and b are unlinked; a is 0 m.u. from the centromere, while b is 10 m.u. from the centromere.e. a and b are 0 m.u. apart.f. a and b are 10 m.u. apart. Assume all…arrow_forwardFor a haploid fungus, the starting point in the biosynthesis of the amino acid arginine is Compound X, which is always present in and absorbed from the environment. The arginine biosynthetic pathway is: Enzyme A Enzyme B Enzyme Ç Compound X It is know that genes encoding enzymes A and C are on two different chromosomes. Compound Y Compound Z- Arginine A mutant strain of genotype a (lacking only enzyme A) is crossed to a mutant strain of genotype c (lacking only enzyme C) to generate a diploid strain. Sporulation (i.e. meiosis) is subsequently induced in the resulting diploid strain. What proportion of the spores (haploids formed by sporulation) is expected to grow on medium without arginine but supplemented with Compound Y? O 100% 50% 0% 25%arrow_forward
- One yeast strain carries the alleles lys+ and arg+, whereas another strain has lys-3 and arg-2. The two strains were crossed toeach other, and an ascus obtained from this cross has four spores with the following genotypes: lys+ arg+, lys+ arg-2, lys-3arg+, and lys-3 arg 2. This ascus has a. a parental ditype.b. a tetratype.c. a nonparental ditype.d. either a tetratype or a nonparental ditype.arrow_forwardIn a wild-type fungus, protein E (encoded by the haplosufficient gene E) normally dimerizes to catalyzes a biochemical reaction necessary for the production of a dark pigment. Ed represents a mutant, dominant negative allele of gene E. What is the predicted phenotype of a fungus cell of genotype E*/Ed, and why? O wild type (normal production of the dark pigment), as E is haplosufficient mutant (no pigment production), as no dimers will form in the heterozygous mutant (no pigment production), as the mutant allele Eg is dominant O wild type (normal production of the dark pigment), as dimers of wild-type and mutant protein E will be formed in the heterozygousarrow_forwardJA yeast proline, histidine auxotroph is mated with a prototroph and the diploid cell is sporulated. a.) Write out the cross that generated the diploid cell? b. ) List the types of tetrads and the genotypes of each spore in the tetrad that will occur if this diploid cell is sporulated.arrow_forward
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