Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Chapter 5, Problem 39P
A cross was performed between a yeast strain that requires methionine and lysine for growth (met- lys-) and another yeast strain, which is met+ lys+. One hundred asci were dissected, and colonies were grown from the four spores in each ascus. Cells from these colonies were tested for their ability to grow on petri plates containing either minimal medium (min), min + lysine (lys), min + methionine (met), or min + lys + met. The asci could be divided into two groups based on this analysis:
Group 1: In 89 asci, cells from two of the four spore colonies could grow on all four kinds of media, while the other two spore colonies could grow only on min + lys + met.
Group 2: In 11 asci, cells from one of the four spore colonies could grow on all four kinds of petri plates. Cells from a second one of the four spore colonies could grow only on min + lys plates and on min + lys + met plates. Cells from a third of the four spore colonies could only grow on min + met plates and on min + lys + met. Cells from the remaining colony could only grow on min + lys + met.
| a. | What are the genotypes of each of the spores within the two groups of asci? |
| b. | Are the lys and met genes linked? If so, what is the map distance between them? |
| c. | If you could extend this analysis to many more asci, you would eventually find some asci with a different pattern. For these asci, describe the to form colonies on the four kinds of petri plates. |
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An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.What is the map distance (in minutes) between these two genes?
Baker's yeast, Saccharomyces cerevisiae, is a single-celled, diploid fungus (which is, of course, a eukaryote, that is capable of both meiosis and sexual reproduction).
Wild type yeast can normally grow on solid or liquid minimal medium; you isolate three mutant strains which are no longer capable of growing on minimal medium alone, however, they can grow on medium supplemented with adenine.
All three yeast strains are homozygous for the underlying alleles.
When you cross mutant strain 1 and mutant strain 2, the offspring cannot grow on minimal medium alone and require adenine supplementation;
when you cross mutant strain 1 and mutant strain 3, the offspring can grow on minimal medium alone and do not require adenine.
After crossing the F1 generation of the cross between mutant strains 1 and 3, you count and determine the phenotypes of 1,000 colonies (here a colony is equivalent to an individual): 563 colonies that can grow on minimal medium alone; 437 colonies that require adenine…
Baker's yeast, Saccharomyces cerevisiae, is a single-celled, diploid fungus (which is, of course, a eukaryote, that is capable of both meiosis and sexual reproduction).
Wild type yeast can normally grow on solid or liquid minimal medium; you isolate three mutant strains which are no longer capable of growing on minimal medium alone, however, they can grow on medium supplemented with adenine.
All three yeast strains are homozygous for the underlying alleles.
When you cross mutant strain 1 and mutant strain 2, the offspring cannot grow on minimal medium alone and require adenine supplementation;
when you cross mutant strain 1 and mutant strain 3, the offspring can grow on minimal medium alone and do not require adenine.
A. What conclusions can you make about the alleles of mutant strains 1, 2, and 3 and their relationships with each other?
B. What phenomenon is occurring in the cross between mutant strains 1 and 3?
After crossing the F1 generation of the cross between mutant strains 1…
Chapter 5 Solutions
Genetics: From Genes to Genomes, 5th edition
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