Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 22, Problem 16P
In Fig. 22.14c, the fw2.2 causal gene was validated by introducing the S. pennellii version of the gene into S. lycopersicum. Why was the experiment done this way, rather than the other way around?
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In Figure 5-14, which is the last gene to be transferredinto the F– from each of the five Hfr strains?
Describe a series of experiments to SELECT and/or SCREEN for strains containing mutations that specifically disrupt the function of StyRS/StyAB and not StyC/StyD in Pseudomonas strain Y2 .
Using the technique of interrupted mating, five Hfr strains were tested for the sequence in which they transmitted several different genes to an F- strain. Each Hfr strain was found to transmit its genes in a unique sequence, as follows (only the first six genes transmitted were scored).
Hfr 1 F O P R S Q
Hfr 2 R P O F G Y
Hfr 3 Y X W Q S R
Hfr 4 W Q S R P O
Hfr 5 F G Y X W Q
A. For each of the Hfr strains, state which donor-gene marker should be selected in the recipients after conjugation to obtain the highest proportion of recombinants that will be Hfr?
Strain 1
Strain 2
Strain 3
Strain 4
Strain 5
Chapter 22 Solutions
Genetics: From Genes to Genomes
Ch. 22 - Choose the best matching phrase in the right...Ch. 22 - Suppose you grew genetically identical dandelion...Ch. 22 - How can each of the following be used in...Ch. 22 - Two different groups of scientists studying a rare...Ch. 22 - Which of the following statements would be true of...Ch. 22 - Studies have indicated that for pairs of twins...Ch. 22 - Prob. 7PCh. 22 - Prob. 8PCh. 22 - Table 22.2 lists concordance values for MZ and DZ...Ch. 22 - Prob. 10P
Ch. 22 - Prob. 11PCh. 22 - Two alleles at one locus produce three distinct...Ch. 22 - In a certain plant, leaf size is determined by...Ch. 22 - Compare and contrast the use of SNP genotyping: i...Ch. 22 - Explain the similarities and differences between...Ch. 22 - In Fig. 22.14c, the fw2.2 causal gene was...Ch. 22 - Among the most prevalent pathologies that afflict...Ch. 22 - Human geneticists have found the Finnish...Ch. 22 - Canavan disease, caused by homozygosity for a...Ch. 22 - In GWAS analysis, because of the existence of LD...Ch. 22 - In Fig. 22.15: a. Why do some chromosomes in the...Ch. 22 - Consider the triangle diagram shown in Fig. 22.17....Ch. 22 - Prob. 23PCh. 22 - You conduct a Case/Control study comparing the...Ch. 22 - Prob. 25PCh. 22 - ALS amyotrophic lateral sclerosis is a rare, fatal...Ch. 22 - Through GWAS explorations, scientists have...Ch. 22 - In domesticated dogs, size has a high...Ch. 22 - Suppose a GWAS investigation found a particular LD...Ch. 22 - In 2008, Time magazine named as its invention of...
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- With the technique of interrupted mating four Hfr strains were tested for the sequence in which they transmitted a number of different genes to an F strain. Each Hfr strain was found to transmit its genes is a unique sequence, as shown in the accompanying table. What is the gene sequence in the original strain from which these Hfr strains were derived?arrow_forwardDescribe the experiment done by Frederick Griffith in 1928, where the non-lethal (rough) strain of Streptococcus pneumoniae bacteria killed experimental mice when mixed with the heat-killed smooth strain. Discuss what may have happened to the rough strain after the heat-killed smooth strain was introduced and why you think the rough strain killed the mice. What would be the conclusion and significance of this study and its finding?arrow_forwardIn five Hfr strains, each of which was used to build a time-of-transfer map, the genes entered the recipient cells as follows: Strain 1: S L A C T F Strain 2: N P F T C A Strain 3: T F P N U Y Strain 4: S H Y U N P Strain 5: U N P F T C Which of the following represents a correct gene map of these results? N P F T S L A C H U Y S L A C T F P N H Y U C T F P N U Y H S L A T C A L S P N U Y H F U N P C A L S F T H Yarrow_forward
- What are the two main phenomena responsible for the emergence of new strains of influenza A? Briefly describe how these events develop and contribute to emergence of new strains.arrow_forwardDNA from a strain of Bacillus subtilis with genotype a+ b+ c+ d+ e+ is used to transform a strain with genotype a− b− c− d− e−. Pairs of genes are checked for cotransformation, and the following results are obtained: Pair of genes Cotransformation Pair of genes Cotransformation a+ and b+ No b+ and d+ No a+ and c+ No b+ and e+ Yes a+ and d+ Yes c+ and d+ No a+ and e+ Yes c+ and e+ Yes b+ and c+ Yes d+ and e+ No On the basis of these results, what is the order of the genes on the bacterial chromosome?arrow_forwardHow many COVID-19 cases are there in the world todayarrow_forward
- . Figure 1-7 shows a simplified pathway for argininesynthesis in Neurospora. Suppose you have a specialstrain of Neurospora that makes citrulline but not arginine. Which gene(s) are likely mutant or missing inyour special strain? You have a second strain ofNeurospora that makes neither citrulline nor argininebut does make ornithine. Which gene(s) are mutant ormissing in this strain?arrow_forwardWhat is the benefit of doing a modified Furter-Meyer Test? What is the premise of this experiment? How would you know if this test yielded a positive result?arrow_forwardFour Hfr strains are derived from an F+ strain of E. Coli to serve as donors for an interrupted-mating experiment. Use the time-table and partial map of the F+ strain (shown below) to determine the genes’ respective positions. Keep in mind that the map distances are NOT proportional, only the FIRST 5 markers are indicated per strain, and the entry times, recorded in minutes, are in parentheses. Transferred genes represent wild-type alleles. Based on the data, which gene can be located at position 5 on the map?arrow_forward
- How do you use qPCR to confirm the phenotype of dpy-5 and skn-1. Describe the experiment.arrow_forwardAustin Taylor and Edward Adelberg isolated some new strains of Hfr cells that they then used to map several genes in Escherichia coli by using interrupted conjugation. In one experiment, the researchers mixed cells of Hfr strain AB‑312, which were xyl+ mtl+ mal+ met+ and sensitive to phage T6, with F− strain AB‑531, which was xyl− mtl− mal− met− and resistant to phage T6. The cells were allowed to undergo conjugation. At regular intervals, the researchers removed a sample of cells and interrupted conjugation by killing the Hfr cells with phage T6. The F− cells, which were resistant to phage T6, survived and were then tested for the presence of genes transferred from the Hfr strain. The results of this experiment are shown in the graph. On the basis of these data, give the order of the xyl, mtl, mal, and met genes on the bacterial chromosome and the minimum distances between them in minutes. The origin of transfer is represented by the red triangle. The distances between genes are not…arrow_forwardIf 20% of inactive controls had been misclassified as active? f 10 % of inactive cases had been misclassified as active?arrow_forward
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