Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 4, Problem 12RQ
Summary Introduction
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The overall standard free energy change (
Introduction:
The
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The following question focuses on how the parameters regulating enzyme function might change, and how these might appear graphically on a Michaelis-Menten plot and a Lineweaver-Burke plot. Carbonic anhydrase is an enzyme that will convert CO2 and water into HCO3.
CO2 + H20 > H+ + HCO3
There are many different isoforms of this enzyme. (see for instance
http://en.wikipedia.org/wiki/Carbonic_anhydrase
1 Assume that one variant has a Km of 10 µM and a different variant has a Km of 100 µM. Draw on the same graph a typical Michaelis-Menton plot showing the alteration in the rate of carbonic anhydrase as the CO2 level is varied for the two different variants of enzyme, assuming the concentration of the enzyme (10 mM) in the test tube is kept constant. Assume that you have equal amounts of the two different variants of carbonic anhydrase in a number of test tubes and that the Vmax for both enzymes are the same. Be sure to label the axes. For the same conditions as above, draw a…
The following question focuses on how the parameters regulating enzyme function might change, and how these might appear graphically on a Michaelis-Menten plot and a Lineweaver-Burke plot. Carbonic anhydrase is an enzyme that will convert CO2 and water into HCO3.
CO2 + H20 > H+ + HCO3
There are many different isoforms of this enzyme. (see for instance
http://en.wikipedia.org/wiki/Carbonic_anhydrase . Imidazol is a competitive inhibitor of carbonic anhydrase. It is effective at an alkaline (high) pH; in lower (more acidic) pH, it no longer inhibits the enzyme. Draw on a separate graph a Lineweaver-Burke plot for the effects of this compound at high pH and low pH. Be sure to label the axes and put in sample data points.
The cleavage of fructose-1,6-bisphosphate to glyceraldehyde3-phosphate and dihydroxyacetone phosphate is an exampleof an __________________ reaction
Chapter 4 Solutions
Biochemistry: The Molecular Basis of Life
Ch. 4 - Prob. 1QCh. 4 - Prob. 2QCh. 4 - Prob. 1RQCh. 4 - Prob. 2RQCh. 4 - Prob. 3RQCh. 4 - Prob. 4RQCh. 4 - Prob. 5RQCh. 4 - Prob. 6RQCh. 4 - Prob. 7RQCh. 4 - Prob. 8RQ
Ch. 4 - Prob. 9RQCh. 4 - Prob. 10RQCh. 4 - Prob. 11RQCh. 4 - Prob. 12RQCh. 4 - Prob. 13RQCh. 4 - Prob. 14RQCh. 4 - Prob. 15RQCh. 4 - Prob. 16RQCh. 4 - Prob. 17RQCh. 4 - Prob. 18RQCh. 4 - Prob. 19RQCh. 4 - Prob. 20RQCh. 4 - Prob. 21RQCh. 4 - Prob. 22RQCh. 4 - Prob. 23RQCh. 4 - Prob. 24RQCh. 4 - Prob. 25RQCh. 4 - Prob. 26RQCh. 4 - Prob. 27RQCh. 4 - Prob. 28RQCh. 4 - Prob. 29RQCh. 4 - Prob. 30FBCh. 4 - Prob. 31FBCh. 4 - Prob. 32FBCh. 4 - Prob. 33FBCh. 4 - Prob. 34FBCh. 4 - Prob. 35FBCh. 4 - Prob. 36FBCh. 4 - Prob. 37FBCh. 4 - Prob. 38FBCh. 4 - Prob. 39FBCh. 4 - Prob. 40SACh. 4 - Prob. 41SACh. 4 - Prob. 42SACh. 4 - Prob. 43SACh. 4 - Prob. 44SACh. 4 - Prob. 45TQCh. 4 - Prob. 46TQCh. 4 - Prob. 47TQCh. 4 - Prob. 48TQCh. 4 - Prob. 49TQCh. 4 - Prob. 50TQCh. 4 - Prob. 51TQCh. 4 - Prob. 52TQCh. 4 - Prob. 53TQCh. 4 - Prob. 54TQCh. 4 - Prob. 55TQCh. 4 - Prob. 56TQCh. 4 - Prob. 57TQCh. 4 - Prob. 58TQCh. 4 - Prob. 59TQCh. 4 - Prob. 60TQCh. 4 - Prob. 61TQCh. 4 - Prob. 62TQ
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- UDP-glucuronosyltransferase enzymes bind the organic compound UDP-glucuronic acid (UDP-GA) in order to catalyse the transfer of a glucuronic acid group from UDP-GA to a drug molecule, releasing UDP from the active site as a product. UDP is then regenerated by the activity of another enzyme. What terms could be used to describe UDP-GA?arrow_forwardSucrose (common table sugar) is hydrolyzed to glucose and fructose in a classic experiment in kinetics. The reaction is catalyzed by the enzyme invertase. Using the following data, determine, by the Lineweaver-Burk method, whether the inhibition of this reaction by 2 M urea is competitive or noncompetitive.arrow_forwardThe following question focuses on how the parameters regulating enzyme function might change, and how these might appear graphically on a Michaelis-Menten plot and a Lineweaver-Burke plot. Carbonic anhydrase is an enzyme that will convert CO2 and water into HCO3. CO2 + H20 > H+ + HCO3 There are many different isoforms of this enzyme. Morphine is a non-competitive inhibitor of carbonic anhydrase. Draw on the same Lineweaver-Burke plot as above a graph showing the effect of a concentration of morphine that inhibits the first enzyme such that it reduces the Vmax to ½ its maximal value. Make sure to put in sample data points. Imidazol is a competitive inhibitor of carbonic anhydrase. It is effective at an alkaline (high) pH; in lower (more acidic) pH, it no longer inhibits the enzyme. Draw on a separate graph a Lineweaver-Burke plot for the effects of this compound at high pH and low pH. Be sure to label the axes and put in sample data points.arrow_forward
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