Concept explainers
To answer:
Use synthetic nucleic acid.
Introduction:
The recombinant DNA technology involves isolation of genetic materials (DNA) from donor organisms (human, plant, or microorganism) and inserts them into recipient organisms after genetic manipulation. The main tools involved in the techniques are
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Chapter 8 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
- You are trying to determine the base content for a number of samples in the lab (mouse DNA, bacterial DNA, single-stranded DNA virus and double-stranded RNA virus). Unfortunately, you failed to use an alcohol-proof marker and during the ethanol precipitation of your samples the labels came off the tubes. You finish the experiment anyway and try to use the data to determine the samples. Can you determine the source of each sample based on your results? Briefly explain how you come to your designations. Sample % adenine % cytosine % guanine % thymine % uracil A 18 32 32 18 0 B 26 18 32 24 0 C 31 19 19 0 31 D 10 40 40 10 0arrow_forward5) How many different bases are found in DNA a) 1 b) 2 c) 3 d) 4arrow_forward.What methods are used to visualize radioactively labeled nucleic acids?arrow_forward
- Arrangement of nucleotides in DNA can be seen using which of the following instruments? a) Electron microscope b) Light microscope c) X-Ray crystallography d) Ultracentrifugearrow_forwardWhat is DNA? Provide a 5-sentence long description only.arrow_forwardGive the meanings of the following terms: genomics, functionalgenomics, and proteomics.arrow_forward
- Watch the demonstration video on nucleic acid quantification on the NanoDrop spectrophotometer: https://cutt.ly/bio150dnaquantification Note: the video is entitled RNA but the method is identical for DNA quantification. 1. At what ratio of A260/280 can we say that DNA is pure? What about RNA and protein?2. While spectrophotometric methods are effective at detecting DNA, a more sensitive but expensive technique called fluorometry is used in sensitive applications. What is the principle behind fluorometry and why is it better than spectrophotometry in detecting DNA?arrow_forwardDescribe the “blender experiments” of Hershey and Chase and what they revealed about DNA versus protein?arrow_forwardDiscuss the benefits of being able to predict protein structure from the DNA sequence (proteomics).arrow_forward
- Please answer all of these questions in details Answer all of the following questions a) Define the following terms: biotechnology, Plasmid,Genetic engineering and Transformation b) List two types of medical products that can be produced using DNA tech- nology c) Relate bioethics to the continued development of genetic engineering techniques. d) Describe two major goals of the Human Genome Project. e) Distinguish between proteomics and genomics. f) Outline the different applications of biotechnology g) List some techniques of genetic engineering. h) Delineate the transgenic organisms and their significance. i) Explain the procedure for the following PCR, Gel electrophpresis and Gene therapy j) Describe human genome project and its main goals. k) Discuss the drastic environmental impacts of oil spills and how the mi- crobes like bacteria can help mitigate the problem. l) Give an account of significance of transgenic organisms in food industry. m) Describe the uses of DNA finger…arrow_forwardBefore X-ray diffraction and the discovery of the structure of deoxyribonucleic acid (DNA) was identified, what historical knowledge and technology existed? Explain previous models, theories and laws and technologies of that time. (Long response)arrow_forwardReview the table below. Nucleic acid from 4 different samples were analyzed to determine the amounts of nitrogenous bases in each. The amount is shown as a percentage. A G T U C Sample 1 22% 28% 22% 28% Sample 2 30% 30% 20% 20% Sample 3 18% 32% 18% 32% Sample 4 29% 29% 21% 21% What observations can you make from these data? Suggest at least two different observations. What conclusions can be supported by these observations? • Suggest one experiment that you could conduct to test or further investigate one of your conclusions from part b.arrow_forward
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