EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Textbook Question
Chapter 8, Problem 3TMW
Why wasn’t polymerase chain reaction (PCR) practical before the discovery of hyperthermophilic bacteria?
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Why is it important to use a hyperthermophilic DNA polymerase in PCR?
a) Because only hyperthermophiles have DNA polymerases.
b) Because hyperthermophilic DNA polymerase is able to resist the saline reaction conditions.
c) Because hyperthermophilic DNA polymerase is faster than other polymerases.
d) Because hyperthermophilic DNA polymerase is able to resist denaturation at 95℃.
What is PCR? why is it important for the manufacture of drugs? How is it used in forensic science?
Was LUCA likely an RNA-based or DNA-based organism? Why?
Chapter 8 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 8 - Why arent the terms recombinant DNA technology...Ch. 8 - Prob. 2TMWCh. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Prob. 5TMWCh. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MC
Ch. 8 - Prob. 6MCCh. 8 - Prob. 7MCCh. 8 - Prob. 8MCCh. 8 - Prob. 9MCCh. 8 - Prob. 10MCCh. 8 - Modified True/False 1. ________ Restriction...Ch. 8 - Modified True/False 2. ________ Restriction...Ch. 8 - Prob. 3MTFCh. 8 - Prob. 4MTFCh. 8 - Prob. 5MTFCh. 8 - Label the reagents and steps of PCR on the figure...Ch. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - Prob. 4SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - CRITICAL THINKING 2 A cancer-inducing virus,...Ch. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - Prob. 4CTCh. 8 - Prob. 5CTCh. 8 - Prob. 6CTCh. 8 - Prob. 7CTCh. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...
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- How will you know if the bacterial cells were transformed during the lab? notes are attached belowarrow_forwardWho isolated Restriction enzymes for the first time?arrow_forwardFor what purpose is DNA fingerprinting used A) to sequence DNA from bacteria B) to separate DNA fragments C) to identify individuals who have committed crimes D) to identify single nucleotide polymerasearrow_forward
- 21) Difference between DNA and RNA 22) PCR 23) RT-PCRarrow_forwardDescribe how would you determine the following using a real-time PCR instrument. A) The quantity and quality of a DNA sample. B) Whether an individual had cystic fibrosis from a genomic DNA sample. C) If several bacterial pathogens were present in DNA extracted from a food sample.arrow_forwardExplain the basis for identification using DNA fingerprinting. – relate this to Microbiology not to human fingerprinting.Why does this technique work? Mention restriction enzymes and their function. Keep answer between 2-3 sentencesarrow_forward
- How does PCR work, and why is it useful?``arrow_forwardExplain the basis for identification using DNA fingerprinting. – relate this to Microbiology not to human fingerprinting. Why does this technique work? Mention restriction enzymes and their function.arrow_forwardWho contributed in the field of microbiology? Each name should only be used once. Developed vaccine against smallpox [ Choose ] Discovered penicillin [Choose ] Disproved spontaneous generation [ Choose ) Was first to observe bacteria [ Choose ] Proved that microorganisms can cause [ Choose ] disease Was first to observe cells and name them [ Choose ] Described serotypes of streptococci [ Choose] Made first recombinant DNA [ Choose ] Studied regulation of bacterial genes [ Choose]arrow_forward
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