Polymerase chain reaction (PCR) is a rapid method involved in the amplification of a specific segment of the DNA molecule. This technique is used to generate a billion copies of the identical molecule of the DNA segment within a few hours (nearly 30 cycles). It is used for clinical studies and the evaluation of genomic variations. The important components used in PCR reactions are DNA, primer, deoxyribonucleotide triphosphates (A, T, G, and C) or dNTPs, Taq DNA polymerase, MgCl2, and buffer. Denaturation (separation of double-stranded DNA into two single-strands of DNA), annealing (binding of primers to ends of single-stranded DNA), and extension (addition of dNTPs at 3’ site of the primer by DNA polymerase) are the three main steps of PCR in each cycle. The amplified products are analyzed using electrophoresis technique.

Question

Want to see the full answer?

Answer 1

Question

Chapter 8, Problem 3MTF

Summary Introduction

Introduction:

Polymerase chain reaction (PCR) is a rapid method involved in the amplification of a specific segment of the DNA molecule. This technique is used to generate a billion copies of the identical molecule of the DNA segment within a few hours (nearly 30 cycles). It is used for clinical studies and the evaluation of genomic variations. The important components used in PCR reactions are DNA, primer, deoxyribonucleotide triphosphates (A, T, G, and C) or dNTPs, Taq DNA polymerase, MgCl2, and buffer. Denaturation (separation of double-stranded DNA into two single-strands of DNA), annealing (binding of primers to ends of single-stranded DNA), and extension (addition of dNTPs at 3’ site of the primer by DNA polymerase) are the three main steps of PCR in each cycle. The amplified products are analyzed using electrophoresis technique.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

CRITICAL THINKING 1. Nitrogen deficiency stunts plant growth and causes leaves to turn yellow and then die. Why does nitrogen deficiency cause these symptoms? Hint: Think about which biological molecules incorporate nitrogen atoms.

Show your solution please. 4.

10_UG_26563_ch09_167-184.indd 175 Press esc to exit full screen Yeast Fermentation The unicellular fungus Saccharomyces cerevisiae is used in the food industry to ferment sugars. The rate of fermentation is measured by CO₂ production. The addition of inorganic phosphate increases this rate. THINK Answer this question individually: 1. Why does the fermentation of sugars, such as glucose and fructose, depend upon the presence of P? PAIR Answer the following questions with a partner: 2. Which glycolytic reactions require P? 3. Several glycolytic intermediates contain phosphate. Where did these groups come from during the reactions? SHARE 4. How much P, is required to generate one CO₂? As a class, discuss the following questions: 5. Is CO₂ generation the most reliable way to measure ethanol fermentation? Why or why not? HANDOUT 175 23/03/17 2:02 pm Page 1 of 4 ...... ZOOM + K