Recombinant DNA technology involves the isolation of genetic materials (DNA) from donor organisms (human, plant, or microorganism) and inserts them into recipient organisms after genetic manipulation. This technology is used to introduce genetically-modified organisms (GMO). The main goals of this technology are to provide (i) elimination of undesirable phenotypic traits in organisms, (ii) creation of new organisms by combining two or more beneficial traits of donor organisms, and (iii) synthesis of beneficial products by the new organism for human needs. Three methods are used for the introduction of genetic material (or DNA) into recipient organisms, namely electroporation, protoplast fusion, and injection.

Question

Want to see the full answer?

Answer 1

Question

Chapter 8, Problem 4MTF

Summary Introduction

Introduction:

Recombinant DNA technology involves the isolation of genetic materials (DNA) from donor organisms (human, plant, or microorganism) and inserts them into recipient organisms after genetic manipulation. This technology is used to introduce genetically-modified organisms (GMO). The main goals of this technology are to provide (i) elimination of undesirable phenotypic traits in organisms, (ii) creation of new organisms by combining two or more beneficial traits of donor organisms, and (iii) synthesis of beneficial products by the new organism for human needs. Three methods are used for the introduction of genetic material (or DNA) into recipient organisms, namely electroporation, protoplast fusion, and injection.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

I AM TRYING TO IDENTIFY THIS UNKNOWN. IMAGE 1 HAS TWO PICTURE OF CATALASE TEST AND BLOOD AGAR TEST. I BELIEVED THAT THIS IS " S. PYOGENES. SO I DID A BACITRACIN TEST ON IT. IMAGE 2 SHOWS BACITRACIN TEST IMAGE. ** PLEASE HELP ME IDENTIFY IF THIS IS S. PROGENES AFTER LOOKING AT ALL THE THREE. BACITRACIN SHOULD BE THE CONFIRMATION TEST. IF YOU THINK THAT THIS IS S. PYOGENES AFTER LOOKING AT THE BACITRACIN TEST, THEN PLEAE EXPLAIN HOW DID YOU FIGURE IT OUT FROM THE CONFIRMATION THAT THIS IS S. PYOGENESE FROM BACITRACIN TEST. NEED VALID REASON WHY YOU BELIEVE IT IS S. PYOGENES

Activity 2. You COMPLETE Me! Objective: Identify the methods used for inserting plasmids into the host cells. What you need: pen and paper What to do: On a separate sheet of paper, copy and complete the key points of the methods used to introduce plasmids into the host cells. Choose your answers from the box gene gun biolistics electric shock mammalian cells plasmid insertion by Heat Shock Treatment increase the pore sizes of their plasma membranes (2) competent plasmid DNA Heat Shock electroporation There are three methods on introducing plasmids into the host cells namely: (1)_ (3) Biolistics uses a (4)_ to fire DNA-coated pellets on plant tissues. Plasmid insertion by Heat Shock Treatment is a process wherein target cells undergo a The pretreatment is said to make the cells (6). pretreatment procedure to (5) the introduction of the (7)_ plasmid at about 4°C for about 30 minutes and the plasmids concentrate near the cells during After the pretreatment, the cells are incubated with…

Multiple Choice 1. For double-stranded DNA, which nucleotide base pairing ratio will result to 1? A. C/T B. (A+T)/(G+C) C. A/G D. (A+C)/(G+T) 2. Why are genetic disorders such as hemophilia and Duchenne muscular dystrophy more prevalent in males than females? A. Because they can only be passed on from father to son B. Because they are recessive disorders C. Because they are dominant genetic disorders D. Because they are X-linked recessive disorders