EBK MICROBIOLOGY:W/DISEASES BY BODY...-
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Chapter 8, Problem 6MC
Summary Introduction

Introduction:

The recombinant DNA technology involves isolation of genetic materials (DNA) from donor organisms (human, plant, or microorganism) and inserts them into recipient organisms after genetic manipulation. The main tools involved in the techniques are nucleic acids, restriction enzymes, ligase, vectors, reverse transcriptase, and mutagens. This technology is used to introduce genetically modified organism (GMO). The main goals of this technology are to provide (i) elimination of undesirable phenotypic traits in organisms, (ii) creation of new organisms by combining two or more beneficial traits of donor organisms, and (iii) synthesis of beneficial products by the new organism for human need.

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Part A:  During cloning, the DNA is cut with a restriction enzyme giving it what?   A. more introns   B. sticky ends   C.  a polyA tail   D. a binding site for a transcription factor Part B: If the scientist wanted to have a large number of copies of the gene for use in further study, which technique would the scientist use?   A. PCR   B. epigenetics   C. hybridization   D. gel electrophoresis Part C: If this gene were found to be expressed at different levels in different cells of the same organism, what would be responsible?    A. transcription factor   B. rRNA   C. ribosome   D. RNA polymerase Part D: If a disease were identified as being caused by defects in the cytochrome c gene, then the copy isolated could be used for what?   A. PCR   B. gene therapy   C. stem cell work   D. DNA fingerprinting
What is the principle of the SNP (single nucleotide polymorphisms) in the diagnosis of human diseases?   a. PCR product of a gene is different from the expected one b. The size of a recombinant DNA is different from the expected one c. Mutation of a single base in a gene makes the size of a band digested by specific restriction enzymes different from the expected one d.   The DNA band detected by Southern blot is different from that by Northern blot
What are the sticky ends of the restriction fragments?   Select one: a. The surfaces of sticky ends contain matching base pairs, allowing fragments to splice. b. The surfaces of sticky ends have glue like substance that allow fragments to splice. c. The surfaces of sticky ends contain the exact same nucleotides, allowing fragments to bond. d. The surfaces of sticky ends have velcro like structure, allowing fragments to bond.
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