Introduction:
The recombinant DNA technology involves isolation of genetic materials (DNA) from donor organisms (human, plant, or microorganism) and inserts them into recipient organisms after genetic manipulation. The main tools involved in the techniques are
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EBK MICROBIOLOGY:W/DISEASES BY BODY...-
- Which lab technique can you use to verify? a.Nucleic Acid Purification b. PCR c.SNP Variationarrow_forwardwhich process releases the DNA into the ionic medium? a. homogenization b. deproteination c. precipitation d. all are correctarrow_forwardWhich type of mutation produces the same protein despite a change in the DNA? A. nonsense B. missense C. silent D. frameshiftarrow_forward
- Which would be most useful in the process of lysing a bacterium so that it's DNA could be collected and studied? A. Lysozyme B. Penicillin C Pymoxin B D. Tetracyclinesarrow_forwardWhat is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' directionarrow_forwardWhich step follows the assembly of new DNA strands by DNA polymerase? a.DNA ligase seals any gaps remaining between bases of new DNA. b.Primers base-pair with the exposed single DNA strands. c.Repair enzymes correct potential mutations in the DNA sequence. d.Enzymes unwind and separate the two strands of DNA.arrow_forward
- What is the role of Sodum Dodecyl Sulffate in DNA isolation? A. Precipitate DNA B Make the solution more viscous C Destroy the cell membrane D Maintain ionic strengtharrow_forwardWhich process is most likely to result in a gene providing antibiotic resistance being found in a viral genome? A. Conjugation B. Binary fission C. Transduction D. Transformationarrow_forwardA mutation in which of these proteins will lead to increased mutations in all daughter cells? A. helicase B. topoisomerase C. ligase D. DNA polymerasearrow_forward
- Which DNA fragment will be closest to the top (negative pole) of anelectrophoretic gel?a. 450 bp b. 3,560 bp c. 5 kb d. 1,500 bparrow_forwardWhat is meant by the term DNA replication? a. synthesis of nucleotides b. cell division c. interpretation of the genetic code d. the exact copying of the DNA code into two new moleculesarrow_forwardWhich step follows the assembly of new DNA strands by DNA polymerase? A. Enzymes unwind and separate the two strands of DNA. B. Repair enzymes correct potential mutations in the DNA sequence. C. Primers base-pair with the exposed single DNA strands. D. DNA ligase seals any gaps remaining between bases of new DNA.arrow_forward
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