EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Chapter 8, Problem 2MTF
Modified True/False
2. ________ Restriction enzymes act at specific
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Activity 2. You COMPLETE Me!
Objective: Identify the methods used for inserting plasmids into the host cells.
What you need: pen and paper
What to do: On a separate sheet of paper, copy and complete the key points of the methods used
to introduce plasmids into the host cells. Choose your answers from the box
gene gun
biolistics
electric shock
mammalian cells
plasmid insertion by Heat Shock Treatment
increase the pore sizes of their plasma membranes
(2)
competent
plasmid DNA
Heat Shock
electroporation
There are three methods on introducing plasmids into the host cells namely: (1)_
(3)
Biolistics uses a (4)_ to fire DNA-coated pellets on plant tissues.
Plasmid insertion by Heat Shock Treatment is a process wherein target cells undergo a
The pretreatment is said to make the cells (6).
pretreatment procedure to (5)
the introduction of the (7)_
plasmid at about 4°C for about
30 minutes and the plasmids concentrate near the cells during
After the pretreatment, the cells are incubated with…
Pipetting
1. Explain why a micropipettor is an important instrument in biochemistry labs.
2. Describe the basic components (and function) of a micropipettor.
Bacterial Techniques
1. Define the following.
(a) Serial Dilution
(b) Streak Plating
(c) Spread Plating
Transformation
1. Define bacterial transformation and explain why it is an important method in
biochemistry labs.
2. Describe (figure, narrative) a plasmid and describe the basic components of a
plasmid.
3. What role does CaCl2 play in bacterial transformation?
4. What role does heat shock play in bacterial transformation?
Plasmid Isolation
1. Describe the roles the following play in plasmid purification.
(a) Lysis Buffer
(b) Neutralization Buffer
(c) Elution Buffer
Write TRUE or FALSE. If false, write the word/s that make(s) the statement incorrect.
1.The primosome complex includes primase, helicase and gyrase.
2.In plasmid DNA biotechnology, plasmid inserted with a “correct" DNA sequence is transferred to bacteria for rapid.
Chapter 8 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 8 - Why arent the terms recombinant DNA technology...Ch. 8 - Prob. 2TMWCh. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Prob. 5TMWCh. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MC
Ch. 8 - Prob. 6MCCh. 8 - Prob. 7MCCh. 8 - Prob. 8MCCh. 8 - Prob. 9MCCh. 8 - Prob. 10MCCh. 8 - Modified True/False 1. ________ Restriction...Ch. 8 - Modified True/False 2. ________ Restriction...Ch. 8 - Prob. 3MTFCh. 8 - Prob. 4MTFCh. 8 - Prob. 5MTFCh. 8 - Label the reagents and steps of PCR on the figure...Ch. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - Prob. 4SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - CRITICAL THINKING 2 A cancer-inducing virus,...Ch. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - Prob. 4CTCh. 8 - Prob. 5CTCh. 8 - Prob. 6CTCh. 8 - Prob. 7CTCh. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...
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- Restriction mapping of the delta chromosome I need help with question two pleasearrow_forwardPlease answer fast After the transfer of the F plasmid is complete multiple choice 3 the F+ cell becomes F-. the F- cell becomes F+. F+ cell becomes antibiotic sensitive. the F+ cell expresses an R factor. the F- cell becomes HfRarrow_forwardMutated DNA Sequence #2 T A C G A C C T T G G C G A C G A C T … What’s the mRNA sequence? (Circle the change) What will be the amino acid sequence? Will there likely be effects? What type of mutation is this? ________________________________arrow_forward
- patibility Mode] UE Layout References Mailings Review View A A Aa vA 前、一间刷 一| T AaBbCcDdE AaBE x A Av X, Normal No Spacing Headi co Edit and Save Start your free one month trial of Microsoft 365 or sign in to activate an existing subscription. 9. In human beings, the gene for red-green colorblindness (r) is sex-linked and recessive to its allele for normal vision (R), while the gene for freckles (F) is autosomal and dominant over its allele for nonfreckled (f). A nonfreckled, normal-visioned woman whose father was freckled and colorblind, marries a freckled, colorblind man whose mother was nonfreckled. a. What is the genotype of the woman's father? b. What is the probability that the couple's first child will be a non-freckled, normal visioned girl c. What is the probability that the first two children born to the couple will be freckled and colorblind girls? d. What is the probability that the first child born to the couple will either be a freckled, colorblind boy or a non-freckled,…arrow_forwardוןווד ← Q Lab Report 6 worksheets 314 F22 .DOCX File Edit View Insert Format Tools Help A 100% ¿ Summary Grades for Arysta Visser: 23 x M Uh-oh! There's a problem w X b The restriction EcoRI cleaves X + Untitled spreadsheet - Goog X https://docs.google.com/document/d/1mKY1HIgMPRh1kRDCmX7msBF2yf07-ogT/edit Outline Headings you add to the document will appear here. Normal text Times ... 12 + B I U 2 18. The restriction EcoRI cleaves double-stranded DNA at the sequence 5'-GAATTC-3', the restriction enzyme HindIII cleaves at the sequence 5'-AAGCTT-3', and the restriction enzyme BamHI cleaves at 5'GGATCC-3. An 805 bp circular plasmid is digested with each enzyme individually and then in combination, and the resulting fragment sizes are determined by means of electrophoresis. The results are as follows: 1 Restriction Enzyme(s) EcoRI BamHI HindIII EcoRI and BamHI EcoRI and HindIII BamHI and HindIII 3 Practice ====•=•€ EX Fragment lengths (base pairs) 430 bp, 375 bp 470 bp, 335 bp Lab Report 6…arrow_forwardTrue or False 1. Any time DNA is damaged, a mutation will result 2. The vast majority of DNA damage is caused by man-made chemicals and man-made radiation 3. Typical DNA polymerases can correct (“proofread”) most of their own mistakes Group of answer choicesarrow_forward
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