Concept explainers
To analyze:
Why the positions of
Introduction:
Blotting is the technique routinely used for the detection of
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Genetic Analysis: An Integrated Approach (2nd Edition)
- Choose two genes from Figure 4.6 and draw a graph to represent the change in transcription over time.arrow_forwardCoomassie dye is the equivalent of what component used in analysis of DNA by agarose gel electrophoresis? How do you expect a Coomassie-stained gel to look compared to the result you might expect once you have completed your Western blot?arrow_forwardIn the Western blot shown here, proteins were isolated from redblood cells and muscle cells from two different individuals. Oneindividual was unaffected, and the other suffered from a diseaseknown as thalassemia, which involves a defect in hemoglobin. Theblot was exposed to an antibody that recognizes β globin, whichis one of the polypeptides that constitute hemoglobin. Equal totalamounts of cellular proteins were added to each lane. Explain these results.arrow_forward
- For your Southern Blot, every band showed a reaction with the probe.Describe what happened and how you could correct for it.arrow_forwardSouthern and Northern blotting are powerful molecular biological tools based on hybridization of nucleic acids. How are these techniques the same? How do they differ? Give some specific applications for each blotting technique.arrow_forwardProvide a detailed description and hand drawn figure of each of the following. (1) Mismatch Repair (2) Nucleotide-Excision Repairarrow_forward
- In Colony Polymerase Chain Rxn (PCR), what primers are necessary to identify that the transformants contain the gene of interest with the correct orientation?arrow_forwardantibodies were produced in a rabbit against a 25 KDa human soluble protien. when the rabbit antiserum was used in a western blot of human soluble protiens, it detected 25-KDa protien,but also bound to protiens of 65KDa and 39 KDa.assuming that a pure protien was used to make the antibodies,how do yuo explain the results of the western blot?arrow_forwardWhich of the following pieces of information about our recombinant DHFR protein do you predict to obtain from Western Blot analysis? A) Protein size B) Protein sequence C) Protein functionarrow_forward
- How does CRISPR work? (Not, "What is CRISPR?" although that is important to know, but how does it work?)arrow_forwardDiagram and explain how APEX probes can be used to determine that an individual is CC (homozygous) for a specific G/C SNV. Recall that the genotype of an SNV is identified from the strand shown in NCBI. What color fluorescence will be observed? Also, explain why a left apex probe cannot be used for this SNV. The SNV sequence, on the strand shown in NCBI, and a few nucleotides adjacent to the SNV are below: 5'-------TGT(G/C)CAG------3'arrow_forwardYou run a Western blot to detect Ras in sampe A and sample B. You find that sample B gives a darker band. What is the most appropriate interpretation of this? (a) sample A has more Ras DNA, (b) sample B has more Ras DNA, (c) sample A has more Ras mRNA, (d) sample B has more Ras mRNA, (e) sample A has more Ras protein, (f) sample B has more Ras protein. I understand that those molecules have higher molecular weight should give darker band. As mRNA has higher molecular weight than that of DNA and protein, I wish to pick (d) "sample B has more Ras mRNA" as the best answer. I am not sure if I am right. Please the expert advise.arrow_forward
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