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Concept explainers
To analyze:
In electrophoretic analysis, if the gel contains two samples of individuals from which one is normal HbA and another has hereditary anemia, no difference is found in the electrophoretic mobility.
The reason behind it is to be described.
Introduction:
Electrophoresis is an experimental approach for separation of protein; the separation can be done on the basis of molecular weight of the protein and shape and charge under the electric field. The protein hasa maximum of
In electrophoresis, low molecular weight protein has low resistance, so it moves faster. The protein with high molecular weight moves slowly. Electrophoresis is a convenient device for an examination of the proteome. It permits separation of a variety of proteins; even proteins with minute differences can separate by this technique.
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Chapter 10 Solutions
Genetic Analysis: An Integrated Approach (2nd Edition)
- sickle cell disease and its variants are genetic disorders resulting from the presence of a mutated form of hemoglobin . propose a proper therapeutic strategy to ameliorate or cure this disease.arrow_forwardRelative to the position of hemoglobin A on your gel, where would you expect to find a mutant hemoglobin in which the b-chain glutamic acid residue was replaced by lysine? Sample Wall Number: 1. Normal Hemoglobin- Homoglobin AA 2. Sickle Trait Hemoglobin- Hemoglobin AS 3. Sickle Cell Hemoglobin- Hemoglobin SS 4. The unkniwn hemoglobin sample (showing the mutant sickle cell anemia trait)arrow_forwardWhy is the Erythrocyte Sedimentation Rate ineffective in patients with severe anemia? Identify an appropriate sedimentation test that could be performed alternatively.arrow_forward
- What is the significance and expected visible result of Test for Oxyhemoglobin and Reduced Hemoglobin in Qualitative tests for blood?arrow_forwardUnder what circumstances and how often should a two-point calibration be performed on a blood gas analyzer? A one-point calibration?arrow_forwardYasuo Yamagata, a 63-year-old, experienced severe back pain for several weeks before visiting his family physician. He also complained of fatigue and looked pale. Blood analysis revealed a red blood cell count of 3.2 × 106/μl (normal 4.2–5.0 × 106/μl), a white blood cell count of 2800/μl (normal 5000/μl), a sedimentation rate of 30 mm/h (normal <20 mm/h) and a serum IgG of 4500 mg/dl (normal 600–1500 mg/dl). IgA and IgM levels were well below normal. Skeletal survey showed lytic lesions in vertebrae, ribs and skull. A bone marrow sample revealed 75% infiltration with plasma cells. Elevated protein in urine was confirmed to be Bence-Jones protein (immunoglobulin light chains). The patient was diagnosed with IgG λ multiple myeloma and began an immediate chemotherapy regime. Which of the following would be consistent with this type of malignant tumor of plasma cells? a. Serum IgG is polyclonal. b. Anemia and neutropenia are present as the result of plasma-cell infiltration in the…arrow_forward
- Calculate the value of colour index of the examined blood if the amount of hemoglobin makes 140g/l, the amount of erythrocytes – 4,2×1012/l. Fulfill the tasks A and B:A) Compare the estimated value with the normal one; write the reference ranges for a healthy adult human.B) Make the conclusion to explain what this value indicates about.arrow_forwardin qualitative test in blood, what is the significance and expected visible result of the test for Test for Oxyhemoglobin and Reduced Hemoglobinarrow_forwardWhat is the significant dividing line in Schilling’s Hemogram? List some conditions in Shift to the left and Shift to the right. NOTE: Kindly answer all questions. Thank you!arrow_forward
- Select the hemoglobin variant(s) that are described by each statement. (a) The variant least likely to cause pathological symptoms. (b) The variant(s) likely to show pl values different from that of HbA on an isoelectric focusing gel. (c) The variant most likely to show a decrease in BPG binding and an increase in the overall affinity of the hemoglobin for oxygen. Answer Bank Hb Milwaukee Hb Cowtown Hb Memphis Hb Philly Hb Providence Hb Bibba HbSarrow_forwardState in details, what the key aspects of the “hemofast staining and a differential white blood cell count” technique that are needed for accuracy? And what are the sources of error that could impact your data and interpretation?arrow_forwardWhat are the specific genetic tests used for Beta-Thalassemia?arrow_forward