Genetic Analysis: An Integrated Approach (2nd Edition)
Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 10, Problem 25P

A second strain of dwarf plants has a different mutation of the same gene identified in Problem 24 . In the second strain, plants carrying a copy of the mutant allele produce a DNA restriction fragment of 10 .5 kb, rather than the 7 .5- kb fragment. DNA fragments produced by digestion of DNA from tall carrier plants are shown below in lanes 1 and 2 , fragments from tall progeny of carriers are shown in lanes 3 and 4 , and DNA from dwarf plants is shown in lane 5 .

Chapter 10, Problem 25P, 25. A second strain of dwarf plants has a different mutation of the same gene identified in Problem.

What mutational mechanism is most likely responsible for the production of abnormal DNA fragment length corresponding to this mutant allele? Explain your reasoning.

In comparison to the length of mRNA from the normal allele, will the mRNA from this mutant allele most likely be longer, shorter, or about the same length? Explain your answer.

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QUESTION 11 The location of five deletions have been mapped to a Drosophila chromosome, as shown in the following deletion map. Recessive mutations a, b, c, d and e are known to be located in the same region as the deletions but the order of the mutations on the chromosome is not known. Drosophila Chromosome Deletion 1 Deletion 2 Deletion 3 Deletion 4 Deletion 5 When flies homozygous for the recessive mutation are crossed with flies heterozygous for the deletions, the following results are obtained in which "m" represents recovery of flies with a mutant phenotype and "+" represents recovery of only flies with a wildtype phenotype. Use the data from the table below to determine the order of the genes (a-e) on the chromosome. Mutants Deletions a b C d e 1 + + + + m 2 + m + m 3 m m m m 4 + + m + + 5 m + m m + What is the gene order for these five genes? Please do not separate the letters representing the mutations with spaces or punctuation (e.g. LMNOP)
Transgenic tobacco plants were obtained in which the vector Ti plasmid was designed to insert the gene of interest plus an adjacent kanamycin-resistance gene. The inheritance of chromosomal insertion was followed by testing progeny for kanamycin resistance. Two plants typified the results obtained generally. When plant 1 was backcrossed with wild-type tobacco, 50 percent of the progeny were kanamycin resistant and 50 percent were sensitive. When plant 2 was backcrossed with the wild type, 75 percent of the progeny were kanamycin resistant and 25 percent were sensitive. What must have been the difference between the two transgenic plants? What would you predict about the situation regarding the gene of interest?
Cyndi grows thousands of B. rapa plants from seed and carefully watches for any unusual plants that might be a new mutation. She has been watching a small seedling whose leaves continue to develop, but the stem is not elongating. The result is a cluster, or rosette, of leaves sitting just above the soil surface. After 15 days the plant is beginning to produce flowers but is still a rosette. Cyndi is encouraged and hypothesizes that rosetteis a recessive mutation.  -Describe the first cross she should make and the predicted results. Because B. rapa is an annual plant, the one rosette individual dies. Cyndi would like to continue her study of this mutation, but all od the offspring from her original cross were normal. There are no more plants with the rosette mutation.  -What can Cyndi do to continue her investigation of rosette? What would be the results of these "next steps"? -What results would you expect if Cyndi crosses her new generation of rosette plants with a known heterozygous…
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