Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Textbook Question
Chapter 10, Problem 26P
During gel electrophoresis of linear DNA molecules, why do longer molecules move more slowly than shorter molecules? What determines the difference in electrophoretic mobility of mRNA molecules?
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Students have asked these similar questions
If mature eukaryotic MRNA is hybridized with its corresponding genomic DNA template strand and
visualized by electron microscopy, two types of structures are seen: RNA:DNA double-stranded
heteroduplexes and single stranded DNA loop structures, as shown in the diagrams below. What do you
think these single stranded DNA loops represent?
(a) Micrograph of DNA-RNA
hybrid
(b) Interpretation of micrograph
Single-stranded
DNA only
Single-stranded DNA
base paired with MRNA
Select one:
а. Exons
b. Introns
c. 5' UTR
d. 3' UTR
e. promoter
During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied?
because DNA is negatively charged
because a charged chemical from the loading buffer is bound to the DNA
because DNA is positively charged
because DNA absorbs electricity
Explain how agarose electrophoresis separates DNA fragments. Why does a smaller DNA fragment move
faster than a larger one?
Chapter 10 Solutions
Genetic Analysis: An Integrated Approach (2nd Edition)
Ch. 10 - Define the following terms as described in this...Ch. 10 - 2. Using sickle cell disease as an example,...Ch. 10 -
3. Compare and contrast the contributions of...Ch. 10 - Why do differences in protein electrophoretic...Ch. 10 - Prob. 5PCh. 10 - Prob. 6PCh. 10 - Prob. 7PCh. 10 - 8. Wildtype βglobin protein is composed of amino...Ch. 10 - Prob. 9PCh. 10 - Prob. 10P
Ch. 10 - 11. How is an autoradiograph produced from a...Ch. 10 - Prob. 12PCh. 10 - Prob. 13PCh. 10 - Prob. 14PCh. 10 - The family represented in the pedigree and...Ch. 10 - Suppose the mating couple (I-1 and I-2) shown in...Ch. 10 - What are restriction endonucleases, and why are...Ch. 10 - 18. Following restriction digestion, DNA fragments...Ch. 10 - 19. The doublestranded DNA sequence below is part...Ch. 10 - 20. Restriction enzymes recognize specific...Ch. 10 - Prob. 21PCh. 10 - Prob. 22PCh. 10 - Prob. 23PCh. 10 - Prob. 24PCh. 10 - 25. A second strain of dwarf plants has a...Ch. 10 - During gel electrophoresis of linear DNA...Ch. 10 - Prob. 27PCh. 10 - 28. In molecular biology, restriction...Ch. 10 - A complete plant gene containing four introns and...Ch. 10 - Prob. 30PCh. 10 - The map below illustrates three alleles in a...Ch. 10 - Prob. 32PCh. 10 - 33. Northern blot analysis is performed on mRNA...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What is the production of RNA called and what is the enzyme that catalyzes the process?What are the similarities and differences between the transcription process and the repli-cation processes?Concerning their biological function what is the difference between DNA and RNA? Is there any situation in which DNA is made based on a RNA template? If there is,explain with an example how it occurs and state the enzyme involved?What is the difference between plasma membrane and cell wall?arrow_forwardWould you expect RNA molecules to behave in the same manner as DNA during gel electrophoresis?arrow_forwardSince RNA polymerase has an error rate of 1 / 10^4 nucleotides, and the DNA polymerase has an error rate of 1 / 10^7 nucleotides, can cells tolerate errors made in transcription in comparison to errors made during DNA replication?arrow_forward
- Why do longer DNA molecules move more slowlythan shorter ones during electrophoresis?arrow_forwardHow is telomerases related to ageing? How Can telomerase- related ageing be addressed therapeutically?arrow_forwardWhat is an Okazaki fragment, and how are they later “glued” together? What are the types and major functions for each type of RNA? Define transcription and translation. Which process occurs first to make protein from DNA? In what direction does a polymerase move when synthesizing a strand of mRNA? What mRNA sequence is synthesized from a section of DNA that is 3’-TTGACCT-5’?arrow_forward
- How can you tell if a protein is stably expressed over time by looking at a pulse-chase analysis gel with bands?arrow_forwardFor human genomic DNA what is the expected fragment size for high molecular weight DNA extracts? What is an important component of gel electrophoresis which is omitted from this result, why would it be important to include ?arrow_forwardRefer to the DNA sequence provided:3’ -TACTGAAGCGGCAGCCCCGCATGAGTAGACCTTACT-5’a. What is the mRNA transcript of the anticoding strand of the DNA model?arrow_forward
- Why do we need to amplify the DNA at all in order to visualize it in a gel?arrow_forwardShown below is an R loop prepared for electron microscopy by annealing a purified eukaryotic messenger RNA with DNA from a genomic clone containing the full-length gene corresponding to the mRNA. (a) How many exons does the gene contain? How many introns? (b) Where in this structure would you expect to find a 5′,5′-internucleotide bond? Where would you expect to find a polyadenylic acid sequence?arrow_forwardChoose 1 or more than 1 RNA are extracted from liver cells and separated in agarose gel by electrophoresis side-by-side with a molecular weight marker. The separated RNA fragments are then transferred to an RNA-binding membrane. Next, this membrane is incubated with labelled probe specific for the gene X. This experiment determines: how many copies of gene X there are in liver cells. if the gene X is translated in liver cells. if gene X has a point mutation in liver cells. the chromosomal location of gene X. the length of the transcript of gene X.arrow_forward
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