a. To determine: The type of suppressor mutations that are expected to arise by using this screen as compared with a screen used for only Lac + . Introduction: The crp gene encodes the positive regulator of CRP. The cells that contain missense mutation in the crp gene include Lac - , Mal - , and Gal - . The cells containing suppressors of the crp mutation behave as if they are crp +. To find these cells, the cells were screened to find those containing Lac+ and Mal+.

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Answer 1

Question

Chapter 15, Problem 16P

Summary Introduction

a.

To determine:

The type of suppressor mutations that are expected to arise by using this screen as compared with a screen used for only Lac⁺.

Introduction:

The crp gene encodes the positive regulator of CRP. The cells that contain missense mutation in the crp gene include Lac^-, Mal^-, and Gal^-. The cells containing suppressors of the crp mutation behave as if they are crp+. To find these cells, the cells were screened to find those containing Lac+ and Mal+.

Summary Introduction

b.

To determine:

The hypothesis that can be proposed based upon the given analysis.

Introduction:

The analysis revealed that all the suppressors that were isolated were found to be mutant in the gene for the α-subunit of RNA polymerase.

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a. Would you expect a cell to continue or to stopdividing at a nonpermissive high temperature if itis a temperature-sensitive Ras mutant whose protein product is fixed in the GTP-bound form atnonpermissive temperature?b. What would you expect if you had a temperaturesensitive mutant in which the Ras protein staysin the GDP-bound form at high temperature?

. Another class of suppressor mutations, not describedin the chapter, are mutations that suppress missensemutations.a. Why would bacterial strains carrying such missense suppressor mutations generally grow moreslowly than strains carrying nonsense suppressormutations?b. What other kinds of mutations can you imagine ingenes encoding components needed for gene expression that would suppress a missense mutationin a protein-coding gene?

Gal4 is a transcription factor that activates transcription of galactose metabolism genes in yeast. These genes are ‘turned on’ when yeast cells need to metabolize galactose. To identify promoter sequences necessary for regulation of transcription of GAL1, reporter gene fusions were made and introduced into yeast cells. Deletions of GAL1 promoter were cloned upstream of LacZ gene. β-Galactosidase activity was measured in presence of galactose. Shown below is a representation of the results obtained. In the diagrams below (not to scale!): • Construct 1 contains ~ 130bp of the promoter, which is predicted to have all the predicted/putative proximal promoter elements (indicated by the solid boxes) needed to regulate transcription of GAL1.• The stippled box is the core promoter.• The arrow represents the transcriptional start site for the reporter gene Lac Z• Number of + signs represents level of transcription• Star represents a mutation in DNA sequence at that location (few nucleotides…

Answer 2

Question

Chapter 15, Problem 16P

Summary Introduction

a.

To determine:

The type of suppressor mutations that are expected to arise by using this screen as compared with a screen used for only Lac⁺.

Introduction:

The crp gene encodes the positive regulator of CRP. The cells that contain missense mutation in the crp gene include Lac^-, Mal^-, and Gal^-. The cells containing suppressors of the crp mutation behave as if they are crp+. To find these cells, the cells were screened to find those containing Lac+ and Mal+.

Summary Introduction

b.

To determine:

The hypothesis that can be proposed based upon the given analysis.

Introduction:

The analysis revealed that all the suppressors that were isolated were found to be mutant in the gene for the α-subunit of RNA polymerase.

Expert Solution & Answer

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Check out a sample textbook solution

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Answer 3

Question

Chapter 15, Problem 16P

Summary Introduction

a.

To determine:

The type of suppressor mutations that are expected to arise by using this screen as compared with a screen used for only Lac⁺.

Introduction:

The crp gene encodes the positive regulator of CRP. The cells that contain missense mutation in the crp gene include Lac^-, Mal^-, and Gal^-. The cells containing suppressors of the crp mutation behave as if they are crp+. To find these cells, the cells were screened to find those containing Lac+ and Mal+.

Summary Introduction

b.

To determine:

The hypothesis that can be proposed based upon the given analysis.

Introduction:

The analysis revealed that all the suppressors that were isolated were found to be mutant in the gene for the α-subunit of RNA polymerase.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 15, Problem 16P

Summary Introduction

a.

To determine:

The type of suppressor mutations that are expected to arise by using this screen as compared with a screen used for only Lac⁺.

Introduction:

The crp gene encodes the positive regulator of CRP. The cells that contain missense mutation in the crp gene include Lac^-, Mal^-, and Gal^-. The cells containing suppressors of the crp mutation behave as if they are crp+. To find these cells, the cells were screened to find those containing Lac+ and Mal+.

Summary Introduction

b.

To determine:

The hypothesis that can be proposed based upon the given analysis.

Introduction:

The analysis revealed that all the suppressors that were isolated were found to be mutant in the gene for the α-subunit of RNA polymerase.

Expert Solution & Answer

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Answer 5

Chapter 15, Problem 16P

Summary Introduction

a.

To determine:

The type of suppressor mutations that are expected to arise by using this screen as compared with a screen used for only Lac⁺.

Introduction:

The crp gene encodes the positive regulator of CRP. The cells that contain missense mutation in the crp gene include Lac^-, Mal^-, and Gal^-. The cells containing suppressors of the crp mutation behave as if they are crp+. To find these cells, the cells were screened to find those containing Lac+ and Mal+.

Summary Introduction

b.

To determine:

The hypothesis that can be proposed based upon the given analysis.

Introduction:

The analysis revealed that all the suppressors that were isolated were found to be mutant in the gene for the α-subunit of RNA polymerase.

Answer 6

Chapter 15, Problem 16P

Summary Introduction

a.

To determine:

The type of suppressor mutations that are expected to arise by using this screen as compared with a screen used for only Lac⁺.

Introduction:

The crp gene encodes the positive regulator of CRP. The cells that contain missense mutation in the crp gene include Lac^-, Mal^-, and Gal^-. The cells containing suppressors of the crp mutation behave as if they are crp+. To find these cells, the cells were screened to find those containing Lac+ and Mal+.

Answer 7

Chapter 15, Problem 16P

Summary Introduction

a.

To determine:

The type of suppressor mutations that are expected to arise by using this screen as compared with a screen used for only Lac⁺.

Introduction:

The crp gene encodes the positive regulator of CRP. The cells that contain missense mutation in the crp gene include Lac^-, Mal^-, and Gal^-. The cells containing suppressors of the crp mutation behave as if they are crp+. To find these cells, the cells were screened to find those containing Lac+ and Mal+.

Answer 8

Summary Introduction

b.

To determine:

The hypothesis that can be proposed based upon the given analysis.

Introduction:

The analysis revealed that all the suppressors that were isolated were found to be mutant in the gene for the α-subunit of RNA polymerase.

Answer 9

Summary Introduction

b.

To determine:

The hypothesis that can be proposed based upon the given analysis.

Introduction:

The analysis revealed that all the suppressors that were isolated were found to be mutant in the gene for the α-subunit of RNA polymerase.

Answer 10

Expert Solution & Answer

Answer 11

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Answer 12

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Answer 13

Ch. 15 - For each of the terms in the left column, choose...Ch. 15 - The following statement occurs early in this...Ch. 15 - One of the main lessons of this chapter is that...Ch. 15 - All mutations that abolish function of the Rho...Ch. 15 - The promoter of an operon is the site to which RNA...Ch. 15 - You are studying an operon containing three genes...Ch. 15 - Prob. 7P Ch. 15 - You have isolated two different mutants reg1 and...Ch. 15 - Bacteriophage , after infecting a cell, can...Ch. 15 - Mutants were isolated in which the constitutive...

Solution Summary: The author explains that all the suppressors that were isolated were found to be mutant in the gene for the -subunit of RNA polymerase.

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b.

To determine:

The hypothesis that can be proposed based upon the given analysis.

Introduction:

The analysis revealed that all the suppressors that were isolated were found to be mutant in the gene for the α-subunit of RNA polymerase.

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Chapter 15 Solutions

Solution Summary: The author explains that all the suppressors that were isolated were found to be mutant in the gene for the -subunit of RNA polymerase.

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b. To determine: The hypothesis that can be proposed based upon the given analysis. Introduction: The analysis revealed that all the suppressors that were isolated were found to be mutant in the gene for the α-subunit of RNA polymerase.

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Chapter 15 Solutions

b.

To determine:

The hypothesis that can be proposed based upon the given analysis.

Introduction:

The analysis revealed that all the suppressors that were isolated were found to be mutant in the gene for the α-subunit of RNA polymerase.