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Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Question
Chapter 15, Problem 29P
Summary Introduction
To determine:
The process by which lacZ fusions can be used in the identification of regulatory genes.
Introduction:
In 2005, it was found that E.coli cells have a global transcription program that helps them in foraging for better sources of carbon. In the experiment, many genes like the one needed for bacterial motility were turned on in response to poorer carbon sources so that the bacteria can search for better nutrition.
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Students have asked these similar questions
The amino acid asparagine is synthesized from aspartic acid by the enzyme asparagine synthetase (AS).
In the previous problem you proposed a model for how this gene could be regulated. Suppose that you carry out an experiment to test your model. To do this you cut out the regulatory sequences upstream of the gene and fuse it to a gene for green fluorescent protein (GFP). Now you can visually observe when the gene is activated. You insert this engineered gene into a host cell and look for GFP expression. You discover some mutants that have different expression levels of GFP and call them GFP1- and GFP2-. The expression levels of GFP are given below.
Cell
GFP expression
Wild type
100
GFP1-
50
GFP2-
0
Propose an explanation for these results based on your model. In other words, what was mutated and how?
Your answer should include whether the mutation is (see links for more information):
dominant or recessive https://www.ncbi.nlm.nih.gov/books/NBK21578/#A1877…
The amino acid asparagine is synthesized from aspartic acid by the enzyme asparagine synthetase (AS).
In the previous problem you proposed a model for how this gene could be regulated. Suppose that you carry out an experiment to test your model. To do this you cut out the regulatory sequences upstream of the gene and fuse it to a gene for green fluorescent protein (GFP). Now you can visually observe when the gene is activated. You insert this engineered gene into a host cell and look for GFP expression. You discover some mutants that have different expression levels of GFP and call them GFP1- and GFP2-. The expression levels of GFP are given below.
Cell
GFP expression
Wild type
100
GFP1-
50
GFP2-
0
Propose an explanation for these results based on your model. In other words, what was mutated and how?
This answer should include whether the mutation is (view links for more information):
dominant or recessive https://www.ncbi.nlm.nih.gov/books/NBK21578/#A1877
in a cis…
cAMP binds to cAMP Receptor Protein (CRP), allowing CRP to bind to the promoter of the lac operon
a) in positive gene regulation by increasing the transcription when glucose is absent and lactose is present
b) in negative gene regulation by decreasing the transcription when glucose is absent and lactose is present
c) in positive gene regulation by increasing the transcription when glucose is present and lactose is absent
d) in negative gene regulation by decreasing the transcription when glucose is present and lactose is absent
Chapter 15 Solutions
Genetics: From Genes to Genomes, 5th edition
Ch. 15 - For each of the terms in the left column, choose...Ch. 15 - The following statement occurs early in this...Ch. 15 - One of the main lessons of this chapter is that...Ch. 15 - All mutations that abolish function of the Rho...Ch. 15 - The promoter of an operon is the site to which RNA...Ch. 15 - You are studying an operon containing three genes...Ch. 15 - Prob. 7PCh. 15 - You have isolated two different mutants reg1 and...Ch. 15 - Bacteriophage , after infecting a cell, can...Ch. 15 - Mutants were isolated in which the constitutive...
Ch. 15 - For each of the E. coli strains containing the lac...Ch. 15 - For each of the growth conditions listed, what...Ch. 15 - For each of the following mutant E. coli strains,...Ch. 15 - Maltose utilization in E. coli requires the...Ch. 15 - Seven E. coli mutants were isolated. The activity...Ch. 15 - Prob. 16PCh. 15 - Six strains of E.coli mutants 16 that had one of...Ch. 15 - a. The original constitutive operator mutations in...Ch. 15 - Prob. 19PCh. 15 - Figure 15.16 on p. 525 shows that in the lac...Ch. 15 - Why is the trp attenuation mechanism unique to...Ch. 15 - a. How many ribosomes are required at a minimum...Ch. 15 - The following is a sequence of the leader region...Ch. 15 - For each of the E. coli strains that follow,...Ch. 15 - One mechanism by which antisense RNAs act as...Ch. 15 - For each element in the list that follows,...Ch. 15 - Great variation exists in the mechanisms by which...Ch. 15 - Many genes whose expression is turned on by DNA...Ch. 15 - Prob. 29PCh. 15 - The E.coli MalT protein is a positive regulator of...Ch. 15 - Prob. 31PCh. 15 - To find genes that are turned on or off in...Ch. 15 - Prob. 33PCh. 15 - Prob. 34PCh. 15 - Prob. 35PCh. 15 - The researchers who investigated bioluminescence...Ch. 15 - Prob. 37PCh. 15 - Quorum sensing controls the expression of...
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