Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Chapter 15, Problem 2P
The following statement occurs early in this chapter: “……a crucial step in the regulation of many bacterial genes is the binding of RNA polymerase to DNA at the promoter.” Why might it be advantageous for bacteria to regulate the expression of their genes at this particular step?
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The following statement occurs early in this chapter:“. . . a crucial step in the regulation of many bacterialgenes is the binding of RNA polymerase to DNA atthe promoter.” Why might it be advantageous forbacteria to regulate the expression of their genesat this particular step?
. One mechanism by which antisense RNAs act as negative regulators of gene expression is by base pairingwith the ribosome binding site on the sense mRNA toblock translation. In a second, alternative mechanism,the act of transcribing an antisense RNA can somehow prevent RNA polymerase from recognizing thesense promoter for the same gene. Design an experimental approach that would enable you to distinguishbetween these two modes of action at a specific gene.(Hint: What would be the outcome in each case ifhigh levels of the antisense RNA were transcribedfrom a gene on a plasmid?)
Negative supercoiling of DNA favors the transcription of genes because it facilitates unwinding. However, not all promoter sites are stimulated by negative supercoiling. The promoter site for topoisomerase II itself is a noteworthy exception. Negative supercoiling decreases the rate of transcription of this gene. Propose a possible mechanism for this effect and suggest a reason why it may occur.
Chapter 15 Solutions
Genetics: From Genes to Genomes, 5th edition
Ch. 15 - For each of the terms in the left column, choose...Ch. 15 - The following statement occurs early in this...Ch. 15 - One of the main lessons of this chapter is that...Ch. 15 - All mutations that abolish function of the Rho...Ch. 15 - The promoter of an operon is the site to which RNA...Ch. 15 - You are studying an operon containing three genes...Ch. 15 - Prob. 7PCh. 15 - You have isolated two different mutants reg1 and...Ch. 15 - Bacteriophage , after infecting a cell, can...Ch. 15 - Mutants were isolated in which the constitutive...
Ch. 15 - For each of the E. coli strains containing the lac...Ch. 15 - For each of the growth conditions listed, what...Ch. 15 - For each of the following mutant E. coli strains,...Ch. 15 - Maltose utilization in E. coli requires the...Ch. 15 - Seven E. coli mutants were isolated. The activity...Ch. 15 - Prob. 16PCh. 15 - Six strains of E.coli mutants 16 that had one of...Ch. 15 - a. The original constitutive operator mutations in...Ch. 15 - Prob. 19PCh. 15 - Figure 15.16 on p. 525 shows that in the lac...Ch. 15 - Why is the trp attenuation mechanism unique to...Ch. 15 - a. How many ribosomes are required at a minimum...Ch. 15 - The following is a sequence of the leader region...Ch. 15 - For each of the E. coli strains that follow,...Ch. 15 - One mechanism by which antisense RNAs act as...Ch. 15 - For each element in the list that follows,...Ch. 15 - Great variation exists in the mechanisms by which...Ch. 15 - Many genes whose expression is turned on by DNA...Ch. 15 - Prob. 29PCh. 15 - The E.coli MalT protein is a positive regulator of...Ch. 15 - Prob. 31PCh. 15 - To find genes that are turned on or off in...Ch. 15 - Prob. 33PCh. 15 - Prob. 34PCh. 15 - Prob. 35PCh. 15 - The researchers who investigated bioluminescence...Ch. 15 - Prob. 37PCh. 15 - Quorum sensing controls the expression of...
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- Some prion-related diseases, such as familial fatal insomnia, areinherited. How would you expect the mutation has altered the PrPgene in this case? Would it have affected the promoter, the regulatorysequences, or the coding sequence of the PrP gene?arrow_forwardDiscuss how the expression of a protein can be regulated post transcription in eukaryotic cells through, using the following key terms: Degradation of mRNA (two ways) Blocking translation Degradation of the proteinarrow_forwardWhat would happen to the regulation of the tryptophan operon in bacterial cells that express a mutant form of the tryptophan repressor that (1) cannot bind to DNA, (2) cannot bind tryptophan, or (3) binds to DNA even in the absence of tryptophan?arrow_forward
- There are similarities and differences during regulation of gene expression in both prokaryotes and eukaryotes. Promoters, transcription factors and RNA polymerase are essential elements in transcription but their properties and function may differ.a) Predict the outcome or consequences of mRNA transcription by RNA polymerase II in eukaryote without the presence of transcription factors (TF).arrow_forwardCompare the control of gene regulation in eukaryotes and prokaryotes at the level of initiation of transcription. How do the regulatory mechanisms work? What are the similarities and differences in these two types of organisms in terms of the specific components of the regulatory mechanisms? Address how the differences or similarities relate to the biological context of the control of gene expression.arrow_forwardAnswer these questions concerning promoters. a) What role do promoters play in transcription? b) What is the common structure of bacterial promoter with respect to consensus sequences? c) Eukaryotic promoters are more variable than bacterial promoters. Why? d) What is the meaning of the term alternative promoter? How does the use of alternative promoters affect transcription?arrow_forward
- The DNA sequence of the promoter region of E. coli xyzA gene is shown below. Transcription start site is the A (in bold) at position 43. 5 10 15 20 25 30 35 40 45 50 GAGCT GTTGA CAATT AATCA TCGAA CTAGT TAACT AGTAC GCAAG TTCAC Mutations were introduced in the sequence to identify residues important for gene expression. Indicate the effect of the following mutations on xyzA expression (increase, decrease, no effect, cannot be predicted). Provide reasoning for each answer. A. G3A (G at position 3 was changed to A) G9A Deletion of TCA at position 18-20 C22A T31A, A32T double mutant T35G G45C C48A B. What are the promoter sequences of the gene?arrow_forwardTranscriptional regulators are proteins that bind to promoters (the 5-flanking regions of genes) to regulate their transcription. Assume that a particular transcription regulator normally promotes transcription of gene X, a transport protein. If a mutation makes this regulator gene nonfunctional, would the resulting phenotype be similar to a mutation in gene X itself? Why or why not?arrow_forwardBacterial DNA containing an operon encoding three enzymes is introduced into chromosomal DNA in yeast (a eukaryote) in such a way that it is properly flanked by a promoter and a transcriptional terminator. The bacterial DNA is transcribed and the RNA correctly processed, but only the protein nearest the promoter is produced. Can you suggest why?arrow_forward
- You are teaching a class on the regulation of eukaryotic gene expression. In order to demonstrate this complex process, you decide to draw for the class a typical eukaryotic gene/transcription unit with its major regions, such as the promoter regions, where the RNA polymerase II and transcription factors would bind From the list given - choose all components that you think are part of a typical eukaryotic gene From the list given - choose all the regulatory sequences that you think would control the expression of this eukaryotic gene From the list given - choose all of the regulatory proteins that would bind the eukaryotic gene to control its expressionarrow_forwardennar region of gene X, which determines the length of the tail in mice, is mutated so that transcription factors bind it at a much higher affinity compared to the wild-type sequence. What is the most likely phenotypic outcome? Tail length will not change because the enhancer is a non-coding sequence Tail length will increased due to increased activity of the gene's promoter Tail length will decreased because any mutation will cause a loss-of-function of these regulatory regions Not just the tail will be enlarged because increased activity of the enhancer will impact many genesarrow_forwardA bacterial species has a hypothetical sigma promoter that has the following sequence: TTGGCA - 18 bases - TATAAT What change in the level of transcription would there be if the sequence was mutated to: TTCGCA -18 bases -TATAAT Group of answer choices 1.The mutation would inhibit the promoter thereby inhibiting transcription 2.No change the consensus TATAAT sequence in the same. 3.The mutation would move the promoter away from consensus and reduce the level of transcription 4.The mutation would bind the promoter to the consensus and produce normal levels of transcriptionarrow_forward
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