a. To determine: The conclusion that can be made regarding the location of the operator site. Introduction: In an experiment, a series of deletions were made to determine the location of an operator site for a negatively regulated gene. The line underneath the sequence shows the extent of each deletion.

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Answer 1

Question

Chapter 15, Problem 19P

Summary Introduction

a.

To determine:

The conclusion that can be made regarding the location of the operator site.

Introduction:

In an experiment, a series of deletions were made to determine the location of an operator site for a negatively regulated gene. The line underneath the sequence shows the extent of each deletion.

Summary Introduction

b.

To determine:

The reason behind no expression of the gene after deletion 2 and 4.

Introduction:

In deletion 2 and 4, the promoter and operator regions are removed. As a result, RNA polymerase does not get a place to bind and move ahead.

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For the trp operon, determine whether genes will be expressed (on) or silenced (off) given the following: a. Tryptophan levels are high. b. The trp repressor can no longer bind tryptophan.

You have isolated two different mutants (reg1 and reg2) causing constitutive expression of the emu operon (emu1 emu2). One mutant contains a defect in a DNA-binding site, and the other has a loss-of-function defect in the gene encoding a protein that binds to the site. Is the DNA-binding protein a positive or negative regulator of gene expression? Explain. To determine which mutant has a defect in the site and which one has a mutation in the binding protein, you decide to do an analysis using F′ plasmids. Assuming you can assay levels of the Emu1 and Emu2 proteins, what results do you predict for the two strains (i and ii; see descriptions below) if reg2 encodes the regulatory protein and reg1 is the regulatory site? Explain. F′ (reg1− reg2+ emu1− emu2+)/reg1+ reg2+ emu1+ emu2− F′ (reg1+ reg2− emu1− emu2+)/reg1+ reg2+ emu1+ emu2−

Explain the process of attenuation in the trp operon under the following conditions: (i) No tryptophan (ii) High tryptophan levels

Answer 2

Question

Chapter 15, Problem 19P

Summary Introduction

a.

To determine:

The conclusion that can be made regarding the location of the operator site.

Introduction:

In an experiment, a series of deletions were made to determine the location of an operator site for a negatively regulated gene. The line underneath the sequence shows the extent of each deletion.

Summary Introduction

b.

To determine:

The reason behind no expression of the gene after deletion 2 and 4.

Introduction:

In deletion 2 and 4, the promoter and operator regions are removed. As a result, RNA polymerase does not get a place to bind and move ahead.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 15, Problem 19P

Summary Introduction

a.

To determine:

The conclusion that can be made regarding the location of the operator site.

Introduction:

In an experiment, a series of deletions were made to determine the location of an operator site for a negatively regulated gene. The line underneath the sequence shows the extent of each deletion.

Summary Introduction

b.

To determine:

The reason behind no expression of the gene after deletion 2 and 4.

Introduction:

In deletion 2 and 4, the promoter and operator regions are removed. As a result, RNA polymerase does not get a place to bind and move ahead.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 15, Problem 19P

Summary Introduction

a.

To determine:

The conclusion that can be made regarding the location of the operator site.

Introduction:

In an experiment, a series of deletions were made to determine the location of an operator site for a negatively regulated gene. The line underneath the sequence shows the extent of each deletion.

Summary Introduction

b.

To determine:

The reason behind no expression of the gene after deletion 2 and 4.

Introduction:

In deletion 2 and 4, the promoter and operator regions are removed. As a result, RNA polymerase does not get a place to bind and move ahead.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 5

Chapter 15, Problem 19P

Summary Introduction

a.

To determine:

The conclusion that can be made regarding the location of the operator site.

Introduction:

In an experiment, a series of deletions were made to determine the location of an operator site for a negatively regulated gene. The line underneath the sequence shows the extent of each deletion.

Summary Introduction

b.

To determine:

The reason behind no expression of the gene after deletion 2 and 4.

Introduction:

In deletion 2 and 4, the promoter and operator regions are removed. As a result, RNA polymerase does not get a place to bind and move ahead.

Answer 6

Chapter 15, Problem 19P

Summary Introduction

a.

To determine:

The conclusion that can be made regarding the location of the operator site.

Introduction:

In an experiment, a series of deletions were made to determine the location of an operator site for a negatively regulated gene. The line underneath the sequence shows the extent of each deletion.

Answer 7

Chapter 15, Problem 19P

Summary Introduction

a.

To determine:

The conclusion that can be made regarding the location of the operator site.

Introduction:

In an experiment, a series of deletions were made to determine the location of an operator site for a negatively regulated gene. The line underneath the sequence shows the extent of each deletion.

Answer 8

Summary Introduction

b.

To determine:

The reason behind no expression of the gene after deletion 2 and 4.

Introduction:

In deletion 2 and 4, the promoter and operator regions are removed. As a result, RNA polymerase does not get a place to bind and move ahead.

Answer 9

Summary Introduction

b.

To determine:

The reason behind no expression of the gene after deletion 2 and 4.

Introduction:

In deletion 2 and 4, the promoter and operator regions are removed. As a result, RNA polymerase does not get a place to bind and move ahead.

Answer 10

Expert Solution & Answer

Answer 11

Expert Solution & Answer

Answer 12

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Answer 13

Ch. 15 - For each of the terms in the left column, choose...Ch. 15 - The following statement occurs early in this...Ch. 15 - One of the main lessons of this chapter is that...Ch. 15 - All mutations that abolish function of the Rho...Ch. 15 - The promoter of an operon is the site to which RNA...Ch. 15 - You are studying an operon containing three genes...Ch. 15 - Prob. 7P Ch. 15 - You have isolated two different mutants reg1 and...Ch. 15 - Bacteriophage , after infecting a cell, can...Ch. 15 - Mutants were isolated in which the constitutive...

Solution Summary: The author analyzes how deletions determine the location of an operator site for a negatively regulated gene.

Concept explainers

Videos

b.

To determine:

The reason behind no expression of the gene after deletion 2 and 4.

Introduction:

In deletion 2 and 4, the promoter and operator regions are removed. As a result, RNA polymerase does not get a place to bind and move ahead.

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Chapter 15 Solutions

Solution Summary: The author analyzes how deletions determine the location of an operator site for a negatively regulated gene.

Concept explainers

Videos

b. To determine: The reason behind no expression of the gene after deletion 2 and 4. Introduction: In deletion 2 and 4, the promoter and operator regions are removed. As a result, RNA polymerase does not get a place to bind and move ahead.

Want to see the full answer?

Chapter 15 Solutions

b.

To determine:

The reason behind no expression of the gene after deletion 2 and 4.

Introduction:

In deletion 2 and 4, the promoter and operator regions are removed. As a result, RNA polymerase does not get a place to bind and move ahead.