Biochemistry: The Molecular Basis of Life
Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 16, Problem 52TQ
Summary Introduction

To review:

The reason behind the loss of muscle mass, rather than fa, tn people following a diet in an attempt to reduce weight.

Introduction:

The feeding-fasting cycle is used for self-regulating mechanisms in the body of mammals to obtain nutrients and energy from food. In metabolism, the concentration of substrate plays a significant role.

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The synthesis of enzymes in response to changing metabolicneeds is referred to as _____________________.
Question 4. Diabetes mellitus possesses a serious health challenge worldwide and there has been an increased awareness for self-monitoring and point of care testing. The development of self- monitoring of blood glucose (SMBG) is probably the most important advance in controlling diabetes since the discovery of insulin. c) Compare the sensitivity and accuracy of glucose meter with a spectrophotometer. d) Elaborate the limitation of glucose meter in diagnostic application? e) Examine the advantage of DNA based biosensor as compared to enzyme biosensor
Question 1. Enzymes, proteins and deoxyribonucleic acid (DNA) are important biological macromolecules. Enzymes are not only speed up the reaction, but also are necessary for DNA reproduction. e) Michaelis - Menten Kinetics model explains how reaction rates are depending on the concentration of enzyme and substrate. i. What is meant by the saturation of enzyme? ii. A decrement in enzyme activity is known as enzyme inhibition. Based on normal enzyme activity, explain the enzyme kinetics for competitive inhibition and noncompetitive inhibition, as shown in Figure 1. Normal enzyme Competitive inhibitor Noncompetitive inhibitor Substrate concentration Figure 1: Enzyme inhibition f) What is the importance of cofactor in the enzyme activity? Give an example of a disease that is related with cofactors. Rate of reaction

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Biochemistry: The Molecular Basis of Life

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