Biochemistry: The Molecular Basis of Life
Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 16, Problem 3RQ
Summary Introduction

To review:

Definition of the following terms:

a. G protein

b. GPCR

c. RTK

d. Growth factor

e. Cytokine

Introduction:

Cell signaling or signal transduction is the transmission of cellular signals from the external environment to inside the cell. It occurs in three different phases, namelyreception, transduction, and response. In the first phase of signal transduction, the cell-surface receptor receives the signal molecule. There are two types of cell-surface receptors, namelyG-proteincoupled receptors and receptor tyrosine kinases.

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Proteins in/on target cells that bind messenger molecules and cause a response in the cells are called _________________.
Question 1. Enzymes, proteins and deoxyribonucleic acid (DNA) are important biological macromolecules. Enzymes are not only speed up the reaction, but also are necessary for DNA reproduction. c) Describe an example of biomedical application that utilizes a protein folding technique. d) Describe the effect of pH on the oxygen binding affinity of hemoglobin.
Question 3. Biochemistry techniques have been used for the analysis on biomolecules such as enzymes, DNA, antibodies and other molecules in biomedical engineering. b) ELISA is a plate-based assay technique, designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones. Examine two principles of ELISA. c) Gel electrophoresis is a laboratory technique, used to separate the mixture of macro- molecules such as DNA, RNA and proteins according to their molecular size. i. Analyze the characteristics of DNA molecule that affect the separation results in the gel electrophoresis. ii. Examine three common factors that are possibly affect the performance of gel electrophoresis.

Chapter 16 Solutions

Biochemistry: The Molecular Basis of Life

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