Biochemistry: The Molecular Basis of Life
Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 6, Problem 57SA
Summary Introduction

To review:

The method by which an investigator can identify the binding of amino acid to the suicide substrate molecule.

Introduction:

An inhibitor is a substance that binds to the active site of an enzyme and hence modifies it. It has a reactive group that reacts irreversibly and results in the formation of a stable inhibitor–enzyme complex. The inhibitor has a functional group that is substituted by a nucleophile in the active site of the enzyme.

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Four important properties of enzymes are high catalytic rate,high degree of substrate specificity, negligible formation ofside products, and _____________________.
If only a few of the amino acid residues of an enzyme are involved in its catalytic activity, then why does the enzyme need such a large number of amino acids?
Suicide substrates are substances that resemble the substrate,but when incorporated into the active site they form a covalent bond with the enzyme, permanently inhibiting it. Thesemolecules have been used to label the amino-acid side chainsthat are involved in the catalytic reaction occurring withinthe active site. This is usually accomplished by hydrolyzingthe inhibited enzyme. How could an investigator identify theamino acid bound to the suicide substrate molecule?

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Biochemistry: The Molecular Basis of Life

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