Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 17, Problem 8CONQ
Together with a specific set of proteins, snoRNAs direct the methylation or pseudouridylation of rRNAs. Does the snoRNA function as a scaffold, guide, ribozyme, blocker, decoy, and/or alterer of protein function or stability?
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Small nucleolar RNAs (snoRNAs) form a large abundant family of noncoding RNAs predominantly localized in the nucleolus. Based on conserved sequence motifs, snoRNAs fall into one of two classes—box C/D snoRNAs or box H/ACA snoRNAs. Each snoRNA associates with a set of class-specific and well-characterized core proteins to form ribonucleoprotein (RNP) complexes referred to as small nucleolar ribonucleoproteins, i.e., box C/D snoRNPs and box H/ACA snoRNPs.
- Gene silencing is mediated by a number of short (~20 nt) noncoding RNAs known as what?
- These noncoding RNAs bind to a protein family that uses single-stranded small nucleic acids as guides to complementary sequences in RNA or DNA for silencing. What protein family is this?
- This RNA-Protein complex attached to DNA forms an overall complex called what?
- The RNA described above doesn’t start out in that form. It is first transcribed in the nucleus as a primary transcript. Describe how this primary transcript is processed and…
An rRNA binds to a snoRNP that contains a C/D box snoRNA.What type of covalent modification would you expect this rRNA toundergo? Would it be methylated or pseudouridylated, or both?
Which type of snoRNA causes an rRNA to be methylated?
Chapter 17 Solutions
Genetics: Analysis and Principles
Ch. 17.1 - Which of the following can bind to ncRNAs? a. DNA...Ch. 17.1 - 2. When an ncRNA functions as a decoy, it
a....Ch. 17.1 - Prob. 3COMQCh. 17.2 - 1. Which of the following functions does HOTAIR...Ch. 17.3 - 1. The process of RNA interference may lead to
a....Ch. 17.3 - 2. In catalyzing the methylation or...Ch. 17.4 - 1. Which of the following is a function of SRP?...Ch. 17.5 - 1. Which of the following components are needed...Ch. 17.5 - 2. In the CRISPR-Cas system, what does the...Ch. 17.5 - Which of the following is a function of the...
Ch. 17.6 - Prob. 1COMQCh. 17.6 - Let's suppose that the overexpression of a...Ch. 17 - 1. List and briefly describe four types of...Ch. 17 - 2. An ncRNA may have the following functions:...Ch. 17 - 3. What is meant by the term RNA world? Describe...Ch. 17 - Explain how HOTAIR plays a role in the...Ch. 17 - What is the phenomenon of RNA interference (RNAi)?...Ch. 17 - With regard to RNAi, what are three possible...Ch. 17 - 7. What is the difference between an miRNA and an...Ch. 17 - Together with a specific set of proteins, snoRNAs...Ch. 17 - Describe the structure of SRP in eukaryotes, and...Ch. 17 - Look at Figure 17.6 and predict what would happen...Ch. 17 - Compare and contrast the roles of crRNA and...Ch. 17 - In the CRISPR-Cas system, does the tracrRNA act as...Ch. 17 - Prob. 13CONQCh. 17 - Outline the steps that occur when piRISCs silence...Ch. 17 - List five types of cancer in which ncRNAs can be...Ch. 17 - Prob. 16CONQCh. 17 - A protein called trypsin, which plays a role in...Ch. 17 - Prob. 2EQCh. 17 - Prob. 3EQCh. 17 - As described in Chapter 21, the CRISPR-Cas system...Ch. 17 - Prob. 5EQCh. 17 - Prob. 6EQCh. 17 - Prob. 1QSDCCh. 17 - Go to the PubMed website and do a search using the...
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- What is TATA box–binding protein (TBP)?arrow_forward. Eukaryotic processing of hnRNA into mature mRNA includes all of the following steps except: ribosome attachment of methionine to the 5’-AUG-3’ codon 5’-addition of a 7-methylguanosine cap 3’-addition of a polyadenylated tail splicing together of exons excision of intronsarrow_forwardWhat are the differences between hnRNAs, snRNAs, miRNAs, siRNAs, and snoRNAs?arrow_forward
- Hetero Nuclear RNA or hnRNA is the unmodified transcript. What process cuts out pieces of this RNA molecule? What is the name of the regions that are cut out?arrow_forwardWhy Cas9 requires a single guide RNA (sgRNA) ?arrow_forwardExplain how snoRNAs direct covalent modifications to rRNAs.arrow_forward
- What is its functional difference with the RNA polymerases found in both eukaryotic and prokaryotic cells?arrow_forwardWhat sequence elements are found within the core promoter of protein-encoding genes in eukaryotes? Describe their locations and specific functions.arrow_forward. Eucaryotic processing of hnRNA into mature mRNA includes all of the following steps except: ribosome attachment to the Shine-Dalgarno sequence 5’-addition of a 7-methylguanosine cap 3’-addition of a polyadenylated tail splicing together of exons excision of intronsarrow_forward
- lin-28 is a gene that is critical for stem cell integrity in mammals and is involved in tumourigenesis, but it was originally identified as a gene involved in developmental timing in C. elegans. It is targeted by the microRNAs lin-4 and let-7 and this affects its translation. Q2a) If the lin-4 sequence is 5’-UCCCUGAGACCUCAAG-3’, which of the following sequences might best correspond to the single target RNA sequence in the 3’UTR of lin-28. A) 3’ UTR- 5’-UCCCUGAGACCUCAAG-3’ B) 3’ UTR- 5’-CUUGAAAUCUCAGUUA-3’ C) 3’ UTR- 5’-CUUGAGGUCUCAGGGA-3’ Q2b) Then describe in 2 sentences/lines why you chose this target sequence.arrow_forwardThe primary transcript for a tRNA molecule has the bases A, U, G, and C. The functionaall TRNA has many other bases, including T, W, I, as result of changes to orriginal bases. How do the changed bases contribute to the 3dimensional structure and function of the TTRNA?arrow_forwardWhich protein subunit specifically binds to the promoter region in prokaryotic cells? α β ω β' σarrow_forward
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