Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 17.5, Problem 2COMQ
In the CRISPR-Cas system, what does the tracrRNA bind to?
a. crRNA and Cas1
b. crRNA and Cas2
c. crRNA and Cas9
d. Cas1 and Cas2
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Chapter 17 Solutions
Genetics: Analysis and Principles
Ch. 17.1 - Which of the following can bind to ncRNAs? a. DNA...Ch. 17.1 - 2. When an ncRNA functions as a decoy, it
a....Ch. 17.1 - Prob. 3COMQCh. 17.2 - 1. Which of the following functions does HOTAIR...Ch. 17.3 - 1. The process of RNA interference may lead to
a....Ch. 17.3 - 2. In catalyzing the methylation or...Ch. 17.4 - 1. Which of the following is a function of SRP?...Ch. 17.5 - 1. Which of the following components are needed...Ch. 17.5 - 2. In the CRISPR-Cas system, what does the...Ch. 17.5 - Which of the following is a function of the...
Ch. 17.6 - Prob. 1COMQCh. 17.6 - Let's suppose that the overexpression of a...Ch. 17 - 1. List and briefly describe four types of...Ch. 17 - 2. An ncRNA may have the following functions:...Ch. 17 - 3. What is meant by the term RNA world? Describe...Ch. 17 - Explain how HOTAIR plays a role in the...Ch. 17 - What is the phenomenon of RNA interference (RNAi)?...Ch. 17 - With regard to RNAi, what are three possible...Ch. 17 - 7. What is the difference between an miRNA and an...Ch. 17 - Together with a specific set of proteins, snoRNAs...Ch. 17 - Describe the structure of SRP in eukaryotes, and...Ch. 17 - Look at Figure 17.6 and predict what would happen...Ch. 17 - Compare and contrast the roles of crRNA and...Ch. 17 - In the CRISPR-Cas system, does the tracrRNA act as...Ch. 17 - Prob. 13CONQCh. 17 - Outline the steps that occur when piRISCs silence...Ch. 17 - List five types of cancer in which ncRNAs can be...Ch. 17 - Prob. 16CONQCh. 17 - A protein called trypsin, which plays a role in...Ch. 17 - Prob. 2EQCh. 17 - Prob. 3EQCh. 17 - As described in Chapter 21, the CRISPR-Cas system...Ch. 17 - Prob. 5EQCh. 17 - Prob. 6EQCh. 17 - Prob. 1QSDCCh. 17 - Go to the PubMed website and do a search using the...
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- Design an experiment, using western blot, to test whether a certain amino acid sequence sends a protein to the ER. Draw what the results of that blot would look like if the sequence sends the protein to the ER and what it would look like if it does not.arrow_forwardexplain what CRISPR/cas13 is and how is it different from CRISPR/cas9, the gene editing toolsarrow_forwardWhich technique allows a researcher to change a single specific nucleotide in a gene sequence in vitro? a. The CRISPR-Cas9 system b. PCR-based random mutagenesis c. PCR-based site-specific mutagenesis d. RT-PCRarrow_forward
- If you want to express a eukaryotic gene in a bacterial cell, you ned to (select all that apply): a.) add a bacterial promoter in front of the gene b.) remove introns from the gene c.) add a polyA tail sequence d.) add eukaryotic tRNAsarrow_forwardPromoters are DNA sequences a. near a transcription start site b. bound to a repressor protein c. that inhibit transcription of a gene d. that stimulate ncRNA activityarrow_forwardDesign an experiment to determine whether a specific gene is transcribed by RNA polymerase II.arrow_forward
- In catalyzing the methylation or pseudouridylation of an rRNA, asnoRNA functions asa. a decoy.b. a scaffold.c. a guide.d. both b and c.arrow_forwardExplain the function of CRISPR in bacterial cells.arrow_forwardA biologist develops a new drug that seems to dramatically slow the onset of symptoms resulting from HIV infection. Close monitoring of HIV-infected cells reveals that the viral proteins are in the form of long polyproteins. What kind of drug did the biologist develop? A. It is a glycosyl transferase inhibitor. B. It is an integrase inhibitor C. It is a protease inhibitor. D. It is a CD4 inhibitor. please explain why which answer is correct and which is wrong and whyarrow_forward
- How do repressors reduce transcription of an operon? A) recruit RNA polymerase to promoters B) block the ability of RNA polymerase to bind a promoter C) break phosphodiester bonds in the DNA D) inhibit the activity of the ribosomearrow_forwardA promoter is ______. a. a specific sequence of DNA nucleotides b. a specific sequence of RNA nucleotides c. a protein that binds to DNA d. an enzyme that synthesizes RNAarrow_forwardThe purpose of site-directed mutagenesis and CRISPR-Cas technologyis toa. determine if a protein binds to a DNA segment.b. determine the sequence of a segment of DNA.c. alter the sequence of a segment of DNA.d. determine if a gene is expressed.arrow_forward
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