Biochemistry: The Molecular Basis of Life
Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 17, Problem 13RQ
Summary Introduction

To review:

Definition of the following terminologies:

1. Hypochromic effect

2. DNA (deoxyribonucleicacid)denaturation

3. Restriction endonucleases

4. DNA hybridization

5. Southern blotting

Introduction:

The structure of DNA is stabilized by the hydrogen bonding between the complementary bases. This bonding is flexible and can be broken and reformed. The complementary nature of DNA bases is also responsible for hybridization and Southern blotting. Moreover, restriction endonucleases can be employed to alter DNA structure.

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Question 1. Although we will not be doing a gel electrophoresis, data from a gel digest of a Bacillus anthrax plasmid is provided so you can do a DNA map. The Bacillus anthrax plasmid is 4000bp (4Kb) long. Note the origin position as well as the reference molecular weight markers on the gel. Two restriction enzymes, A and B, were used to obtain two individual digests, A and B. They were combined to produce the third digest. The restriction enzyme fragment pattern for the digest of Bacillus anthrax plasmid Determining the Number of Fragments How many fragments were produced by enzyme A? How many fragments were produced by enzyme B? How many fragments were produced by the combined digest (A and B)? Fragment Size Fragment size is relative to molecular weight, and must be determined by comparing the fragment distance to the molecular weight markers. The fragment size has been provided on the gel pattern for this exercise. To make a map you must determine the relative positions of the…

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Biochemistry: The Molecular Basis of Life

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