Biochemistry: The Molecular Basis of Life
Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 17, Problem 55SA
Summary Introduction

To review:

Role of polyamines(spermine and spermidine)in enhancing the stability ofDNA(deoxyribonucleic acid)structure and in promoting its supercoiling.

Introduction:

Polyamines, such asspermine and spermidin, ere the polycationic amines that play an important role in bothprokaryotic and eukaryotic cells. They play a role in the processes of transcription, translation, chromatin condensation, and apoptosis due to their interaction with DNA.

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Cytosine can be deaminated to form Uracil   What type of mutation is this classified as? Discuss what happens to the base-pairing properties from switching from C to U? When U is replicated in two rounds of synthesis, what substitution does this result in? Before Uracil alters the DNA during replication, what repair system can be used to correct this error? Describe how this type of DNA repair works?
In the following sequence, a cytosine was deaminated and is now a uracil (underlined). 5’-GGTAUTAAGC-3’ a. Which repair pathway(s) could restore this uracil to cytosine? b. If the uracil is not removed before a DNA replication fork passes through, what will be the sequences of the two resulting double helices? Provide the sequences of both strands of both helices. Label the old and new strands and underline the mutation(s). c. Could the mismatch repair pathway fix the mutations you’ve indicated in part b? d. If the cell undergoes mitosis, and the replicated DNAs are distributed into the two daughter cells. Will 0, 1, or 2 daughter cells have a mutation in this sequence?
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Biochemistry: The Molecular Basis of Life

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