Inflected forms: pl. o's or O's also os or Os 1. The 15th letter of the modern English alphabet. 2. Any of the speech sounds represented by the letter o.3. The 15th in a series. 4. Something shaped like the letter O. 5.O One of the four major blood groups in the ABO system. Individuals with this blood group have neither A nor B antigens on the surface of their red blood cells, and have both anti-A and anti-B antibodies in their blood serum. 6. A zero.